Application | Comment | Organism |
---|---|---|
analysis | use of rosy mutant strains to probe structure and function of xanthine dehydrogenase | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | iron-sulfur groups | Drosophila melanogaster | |
Iron-sulfur-center | substitutions G42E, E89K, L127F located within the iron-sulfur domain | Drosophila melanogaster | |
Mo | - |
Drosophila melanogaster | |
molybdenum-center | substitutions G800E, G1011E, G1164R, G1266D, S1275F located within the pterin molybdenum cofactor domain | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Drosophila melanogaster | i.e. pterin | isoxanthopterin + NADH | - |
? | |
xanthine + NAD+ + H2O | Drosophila melanogaster | - |
urate + NADH | - |
? | |
xanthine + O2 + H2O | Drosophila melanogaster | - |
urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole body | selection of 11 rosy mutant strain, corresponding to single amino acid substitutions for detailed studies on the activities | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
NADH + electron acceptor + H2O | electron acceptors: 2,6-dichlorphenolindophenol or methyl viologen | Drosophila melanogaster | NAD+ + reduced electron acceptor | - |
? | |
NADPH + phenazine methosulfate + cytochrome c | - |
Drosophila melanogaster | NADP+ + ? | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Drosophila melanogaster | urate + ? | - |
? | |
xanthine + NAD+ + H2O | - |
Drosophila melanogaster | urate + NADH | - |
? | |
xanthine + O2 + H2O | - |
Drosophila melanogaster | urate + O2- + 2 H+ | - |
? | |
xanthine + phenazine methosulfate + cytochrome c + H2O | - |
Drosophila melanogaster | urate + ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | substitutions G348E, G353D, S357F located within the flavin/NAD+/NADH-domain | Drosophila melanogaster | |
additional information | - |
Drosophila melanogaster | |
NAD+ | - |
Drosophila melanogaster |