Literature summary for 1.17.1.3 extracted from

Wang, P.; Zhang, L.; Jiang, X.; Dai, X.; Xu, L.; Li, T.; Xing, D.; Li, Y.; Li, M.; Gao, L.; Xia, T.
Evolutionary and functional characterization of leucoanthocyanidin reductases from Camellia sinensis (2018), Planta, 247, 139-154 .

Search for more literature for 1.17.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene LARa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco	Camellia sinensis
gene LARb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco	Camellia sinensis
gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco	Camellia sinensis
gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco	Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+	Camellia sinensis	-	(2R,3S)-catechin + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Camellia sinensis	A0A286QXX3	cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)	-
Camellia sinensis	A0A286QXY4	cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)	-
Camellia sinensis	I1E425	cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Camellia sinensis	-
leaf bud	-	Camellia sinensis	-
additional information	accumulation of catechins is greater in the buds and younger leaves than in the mature leaves, stems and roots	Camellia sinensis	-
root	-	Camellia sinensis	-
stem	young fine shoots	Camellia sinensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+	-	Camellia sinensis	(2R,3S)-catechin + NADP+ + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, about, sequence calculation	Camellia sinensis
?	x * 36000, about, sequence calculation	Camellia sinensis
?	x * 43000, about, sequence calculation	Camellia sinensis

Synonyms

Synonyms	Comment	Organism
CsLAR	-	Camellia sinensis
LARa	-	Camellia sinensis
LarB	-	Camellia sinensis
LarC	-	Camellia sinensis

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Camellia sinensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Camellia sinensis	sequence calculation	-	5.27
Camellia sinensis	sequence calculation	-	5.34
Camellia sinensis	sequence calculation	-	5.43

General Information

General Information	Comment	Organism
evolution	phylogenetic analysis of the LAR family, overview	Camellia sinensis
evolution	phylogenetic analysis of the LAR family, overview. The dicotyledonous LARs can be clustered into two subgroups, which are defined as cluster I and cluster II	Camellia sinensis
malfunction	overexpression of CsLAR causes a decrease in the proanthocyanidins in transgenic plants	Camellia sinensis
physiological function	the enzyme is required in the proanthocyanidin biosynthesis	Camellia sinensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)