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Literature summary for 1.17.1.3 extracted from

  • Pang, Y.; Abeysinghe, I.S.B.; He, J.; He, X.; Huhman, D.
    Mewan, K.M.; Sumner, L.W.; Yun, J.; Dixon, R.A. Functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering (2013), Plant Physiol., 161, 1103-1116 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene LARa, complementary DNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional expression of His6-tagged enzyme in Escherichia coli strain M15. Recombinant ectopic expression of CsLAR leads to the accumulation of low levels of proanthocyanidin precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing Nicotiana tabacum, but levels of oligomeric proanthocyanidins are very low. The expression of CsLAR in tobacco overproducing anthocyanin leads to the accumulation of higher levels of epicatechin and its glucoside than of catechin, phenotype and flavonoid compounds contents, overview Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ Camellia sinensis
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(2R,3S)-catechin + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Camellia sinensis I1E425 cv. TRI2043, blister blight-resistant tea cultivar
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
CsLAR
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Camellia sinensis
LAR
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Camellia sinensis
LARa
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Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Camellia sinensis

pI Value

Organism Comment pI Value Maximum pI Value
Camellia sinensis sequence calculation
-
5.43

General Information

General Information Comment Organism
evolution enzyme LAR is related to members of the reductase-epimerase-dehydrogenase protein superfamily Camellia sinensis