Cloned (Comment) | Organism |
---|---|
gene LARa, complementary DNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional expression of His6-tagged enzyme in Escherichia coli strain M15. Recombinant ectopic expression of CsLAR leads to the accumulation of low levels of proanthocyanidin precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing Nicotiana tabacum, but levels of oligomeric proanthocyanidins are very low. The expression of CsLAR in tobacco overproducing anthocyanin leads to the accumulation of higher levels of epicatechin and its glucoside than of catechin, phenotype and flavonoid compounds contents, overview | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | Camellia sinensis | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | I1E425 | cv. TRI2043, blister blight-resistant tea cultivar | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | - |
Camellia sinensis | (2R,3S)-catechin + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CsLAR | - |
Camellia sinensis |
LAR | - |
Camellia sinensis |
LARa | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Camellia sinensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camellia sinensis | sequence calculation | - |
5.43 |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme LAR is related to members of the reductase-epimerase-dehydrogenase protein superfamily | Camellia sinensis |