Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gottschalkia acidurici | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18400 | - |
x * 99000 (formate dehydrogenase subunit FdhF2) + x * 68000 (HylB) + x * 35900 (HylA) + x * 18400 (HylC), SDS-PAGE, calculated from sequence | Gottschalkia acidurici |
35900 | - |
x * 99000 (formate dehydrogenase subunit FdhF2) + x * 68000 (HylB) + x * 35900 (HylA) + x * 18400 (HylC), SDS-PAGE, calculated from sequence | Gottschalkia acidurici |
68000 | - |
x * 99000 (formate dehydrogenase subunit FdhF2) + x * 68000 (HylB) + x * 35900 (HylA) + x * 18400 (HylC), SDS-PAGE, calculated from sequence | Gottschalkia acidurici |
99000 | - |
x * 99000 (formate dehydrogenase subunit FdhF2) + x * 68000 (HylB) + x * 35900 (HylA) + x * 18400 (HylC), SDS-PAGE, calculated from sequence | Gottschalkia acidurici |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | Gottschalkia acidurici | - |
CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | Gottschalkia acidurici DSM 604 | - |
CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gottschalkia acidurici | K0B3A3 and K0AXR5 and K0B622 and K0B468 | K0B3A3: formate dehydrogenase subunit, K0AXR5: subunit HylA, K0B622: subunit HylB, K0B468: subunit HylC | - |
Gottschalkia acidurici DSM 604 | K0B3A3 and K0AXR5 and K0B622 and K0B468 | K0B3A3: formate dehydrogenase subunit, K0AXR5: subunit HylA, K0B622: subunit HylB, K0B468: subunit HylC | - |
Purification (Comment) | Organism |
---|---|
- |
Gottschalkia acidurici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | - |
Gottschalkia acidurici | CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | ferredoxin is not reduced in the absence of NAD+, and NAD+ is not reduced in the absence of ferredoxin. NADP+ can not substitute for NAD+ | Gottschalkia acidurici | CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | - |
Gottschalkia acidurici DSM 604 | CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? | |
formate + 2 NAD+ + 2 oxidized ferredoxin iron-sulfur cluster | ferredoxin is not reduced in the absence of NAD+, and NAD+ is not reduced in the absence of ferredoxin. NADP+ can not substitute for NAD+ | Gottschalkia acidurici DSM 604 | CO2 + 2 NADH + 2 reduced ferredoxin iron-sulfur cluster | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99000 (formate dehydrogenase subunit FdhF2) + x * 68000 (HylB) + x * 35900 (HylA) + x * 18400 (HylC), SDS-PAGE, calculated from sequence | Gottschalkia acidurici |
Synonyms | Comment | Organism |
---|---|---|
electron-bifurcating formate dehydrogenase | - |
Gottschalkia acidurici |
Fdh/Hyl | - |
Gottschalkia acidurici |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Gottschalkia acidurici |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Fe-S center | the enzyme contains iron-sulfur clusters | Gottschalkia acidurici | |
FMN | enzyme preparation contains FMN | Gottschalkia acidurici | |
NAD+ | NADP+ can not substitute for NAD+ | Gottschalkia acidurici |