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Literature summary for 1.16.99.1 extracted from

  • Ferguson, T.; Soares, J.; Lienard, T.; Gottschalk, G.; Krzycki, J.
    RamA, a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea (2009), J. Biol. Chem., 284, 2285-2295 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.99.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 gel fitlration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a [Co(II) dimethylamine-specific corrinoid protein] + reduced acceptor + H2O Methanosarcina barkeri
-
 ADP + phosphate + a [Co(I) dimethylamine-specific corrinoid protein] + acceptor
-
 ?
ATP + a [Co(II) methylamine-specific corrinoid protein] + reduced acceptor + H2O Methanosarcina barkeri
-
 ADP + phosphate + a [Co(I) methylamine-specific corrinoid protein] + acceptor
-
 ?
ATP + a [Co(II) trimethylamine-specific corrinoid protein] + reduced acceptor + H2O Methanosarcina barkeri
-
 ADP + phosphate + a [Co(I) trimethylamine-specific corrinoid protein] + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri B8Y445
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-

Renatured (Commentary)

Renatured (Comment) Organism
purification from cell-free extract in a Coy anaerobic chamber containing 98% N2 and 2% H2 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a [Co(II) dimethylamine-specific corrinoid protein] + reduced acceptor + H2O
-
 Methanosarcina barkeri ADP + phosphate + a [Co(I) dimethylamine-specific corrinoid protein] + acceptor
-
 ?
ATP + a [Co(II) methylamine-specific corrinoid protein] + reduced acceptor + H2O
-
 Methanosarcina barkeri ADP + phosphate + a [Co(I) methylamine-specific corrinoid protein] + acceptor
-
 ?
ATP + a [Co(II) trimethylamine-specific corrinoid protein] + reduced acceptor + H2O
-
 Methanosarcina barkeri ADP + phosphate + a [Co(I) trimethylamine-specific corrinoid protein] + acceptor
-
 ?
additional information reaction takes place under nitrogen in the absence of hydrogen, but only if Ti(III)-citrate is maintained. Presence of ATP is absolutely required Methanosarcina barkeri ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
RamA
-
 Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center RamA possesses a C-terminal ferredoxin-like domain capable of binding two tetranuclear iron-sulfur proteins Methanosarcina barkeri

General Information

General Information Comment Organism
physiological function RamA is required for in vitro ATP-dependent reductive activation of methylamine:CoM methyl transfer from monomethylamine, dimethylamine, or trimethylamine. In the absence of the methyltransferases, RamA mediates the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the monomethylamine corrinoid protein, MtmC Methanosarcina barkeri