Cloned (Comment) | Organism |
---|---|
gene cyc2, the gene is encoded in the iron oxidation system-encoding rus operon comprising genes cyc1, cyc2, coxA, coxC and rus, quantitative real-time PCR gene expression analysis in strain CCM 4253 | Acidithiobacillus ferrooxidans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Acidithiobacillus ferrooxidans | 19867 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(II) + rusticyanin | Acidithiobacillus ferrooxidans | - |
Fe(III) + reduced rusticyanin | - |
? | |
Fe(II) + rusticyanin | Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 | - |
Fe(III) + reduced rusticyanin | - |
? | |
additional information | Acidithiobacillus ferrooxidans | direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 | ? | - |
? | |
additional information | Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 | direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus ferrooxidans | O33823 | strain CCM 4253 shows 100% identity to type strain ATCC 23270 | - |
Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 | O33823 | strain CCM 4253 shows 100% identity to type strain ATCC 23270 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | cells grown anaerobically on S0 as an electron donor and Fe3+ as an electron acceptor, gene transcript profile of gene cyc2 monitored throughout the cells' growth phases. Reduction in the abundance of Cyc2 in anaerobic cells | Acidithiobacillus ferrooxidans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(II) + rusticyanin | - |
Acidithiobacillus ferrooxidans | Fe(III) + reduced rusticyanin | - |
? | |
Fe(II) + rusticyanin | - |
Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 | Fe(III) + reduced rusticyanin | - |
? | |
additional information | direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 | Acidithiobacillus ferrooxidans | ? | - |
? | |
additional information | direct interaction between rusticyanin and Cyc2, but no interaction between Cyc2 and c4-type cytochrome Cyc1 or c4-type cytochrome CycA1 | Acidithiobacillus ferrooxidans CCM 4253 / ATCC 23270 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cyc2 | - |
Acidithiobacillus ferrooxidans |
outer-membrane iron oxidase | - |
Acidithiobacillus ferrooxidans |
terminal Fe3+ reductase | - |
Acidithiobacillus ferrooxidans |
terminal ferric iron reductase | - |
Acidithiobacillus ferrooxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Acidithiobacillus ferrooxidans | |
rusticyanin | Rus, need for rusticyanin in the anaerobic electron transport pathway | Acidithiobacillus ferrooxidans |
General Information | Comment | Organism |
---|---|---|
malfunction | cells lacking the Fe3+-reducing capacity reveal downregulation of energy metabolism proteins, which are in some cases even absent. Among the repressed and missing proteins are Cyc2, rusticyanin, heterodisulfide reductase (Hdr), thiosulfate:quinone oxidoreductase (Tqo) and sulfide:quinone reductase (Sqr) | Acidithiobacillus ferrooxidans |
metabolism | the enzyme is involved in the involved in the anaerobic pathway of S0 oxidation coupled with dissimilatory Fe3+ reduction. The pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase), periplasmic electron shuttle rusticyanin, c4-type cytochrome CycA1, the inner membrane cytochrome bc1 complex I, and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. An alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Strain- or phenotype-dependent pathway variation, overview. The enzyme is involved in the anaerobic respiratory pathway, regulation of the pathway and model overview | Acidithiobacillus ferrooxidans |
physiological function | ability of Acidithiobacillus ferrooxidans to anaerobically reduce Fe3+, molecular mechanism, overview. Rusticyanin is a stable and highly abundant protein in Fe2+-grown cells, in which it represents about 5% of soluble protein, that fulfills the role of an electron transporter in the respiratory chain, even after strong reduction of its content in a cell. The terminal Fe3+ reductase might be outer membrane cytochrome Cyc2, operating in reverse mode and reducing Fe3+ to Fe2+ under anaerobic conditions | Acidithiobacillus ferrooxidans |