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Literature summary for 1.16.3.1 extracted from

  • Rowland, J.L.; Niederweis, M.
    A multicopper oxidase is required for copper resistance in Mycobacterium tuberculosis (2013), J. Bacteriol., 195, 3724-3373 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.3.1

Protein Variants

Protein Variants Comment Organism
C35A putative lipidation site is dispensable for MmcO activity: mutation shows only minor impact on enzymatic activity Mycobacterium tuberculosis
C486A cysteine 486 is required for MmcO activity. Mutation results in inactive Rv0846c protein which does not protect Mycobacterium tuberculosis against copper stress Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane associated probably by lipidation after export across the inner membrane by the twin-arginine translocation system Mycobacterium tuberculosis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper four copper ions are coordinated in two centers comprise the multicopper oxidase active site Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fe(II) + 4 H+ + O2 Mycobacterium tuberculosis
-
 Fe(III) + 2 H2O
-
 ?
Fe(II) + 4 H+ + O2 Mycobacterium tuberculosis H37Rv
-
 Fe(III) + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis I6WZK7
-
-
Mycobacterium tuberculosis H37Rv I6WZK7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell lysate
-
 Mycobacterium tuberculosis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe(II) + 4 H+ + O2
-
 Mycobacterium tuberculosis Fe(III) + 2 H2O
-
 ?
Fe(II) + 4 H+ + O2
-
 Mycobacterium tuberculosis H37Rv Fe(III) + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MmcO
-
 Mycobacterium tuberculosis
multicopper oxidase
-
 Mycobacterium tuberculosis
mycobacterial multicopper oxidase
-
 Mycobacterium tuberculosis
Rv0846c
-
 Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis expression of the mmcO gene is increased by copper up

General Information

General Information Comment Organism
malfunction deletion of the rv0846c gene increases the susceptibility of Mycobacterium tuberculosis to copper at least 10fold Mycobacterium tuberculosis
physiological function multicopper oxidase is required for copper resistance in Mycobacterium tuberculosis Mycobacterium tuberculosis