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Literature summary for 1.16.3.1 extracted from

  • Butterfield, C.N.; Soldatova, A.V.; Lee, S.W.; Spiro, T.G.; Tebo, B.M.
    Mn(II,III) oxidation and MnO2 mineralization by an expressed bacterial multicopper oxidase (2013), Proc. Natl. Acad. Sci. USA, 110, 11731-11735.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology expression system is developped producing about 2 mg of purified Bacillus sp. strain PL-12 Mn(II) oxidase per liter of Escherichia coli culture in 5 days Bacillus sp. (in: Bacteria)

Cloned(Commentary)

Cloned (Comment) Organism
the mnxDEFG operon construct is expressed without a tag in Escherichia coli by inducing at 17°C and loading with 2 mM CuSO4 under microaerobic conditions Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
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-
-

Purification (Commentary)

Purification (Comment) Organism
using a series of native chromatography steps: hydrophobic interaction, gel filtration, and ion exchange Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 Mn(II) + 4 H+ + O2
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Bacillus sp. (in: Bacteria) 4 Mn(III) + 2 H2O
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?
4 Mn(III) + 4 H+ + O2
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Bacillus sp. (in: Bacteria) 4 Mn(IV) + 2 H2O
-
?
additional information Mn oxidation from soluble Mn(II) to Mn(IV) oxides is a two-step reaction catalyzed by an MCO-containing complex Bacillus sp. (in: Bacteria) ?
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?

Synonyms

Synonyms Comment Organism
mnxDEFG
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Bacillus sp. (in: Bacteria)
multicopper oxidase
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Bacillus sp. (in: Bacteria)