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Literature summary for 1.16.3.1 extracted from

  • Khare, G.; Gupta, V.; Nangpal, P.; Gupta, R.; Sauter, N.; Tyagi, A.
    Ferritin structure from Mycobacterium tuberculosis: Comparative study with homologues identifies extended C-terminus involved in ferroxidase activity (2011), PLoS ONE, 6, e18570.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
determined at 3 A. The crystallographic data implicate the importance of the extended C-terminal region in the iron entry from the three-fold channels to the ferroxidase centre and making iron more readily accessible for the oxidation Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information a truncated protein (having 1-167 amino acids, molecular weight ,18 kDa) is generated: The truncated protein shows a 3.5fold reduction in the oxidation rate of Fe(II). Lack of C-terminus has an impact of the stability of the protein. Truncated BfrB starts unfolding on exposure to even a very low temperature of 30°C whereas the native protein remains almost unaffected till 50°C before denaturing rapidly Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using NH4SO4 precipitation and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 Fe2+ + 4 H+ + O2
-
 Mycobacterium tuberculosis 4 Fe3+ + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
bacterioferritin B
-
 Mycobacterium tuberculosis
BfrB
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 truncated BfrB starts unfolding on exposure to even a very low temperature of 30°C whereas the native protein remains almost unaffected till 50°C before denaturing rapidly Mycobacterium tuberculosis