Crystallization (Comment) | Organism |
---|---|
residues D283, E185, D409 provide a Fe(II) binding site that favors ferric ion thus reducing the reduction potential of the bound Fe(II). Residues E185 and D409 form part of the electron-transfer pathway from the bound Fe(II) to the proteins type I Cu(II) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D283A | wild-type reduction potential | Saccharomyces cerevisiae |
D409A | increase in reduction potential by 120 mV | Saccharomyces cerevisiae |
E185A | wild-type reduction potential | Saccharomyces cerevisiae |
E185A/D409A | increase in reduction potential by 120 mV, complete loss of specificity for Fe(II), functions kinetically as an inefficient laccase | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
Fe(II) | wild-type, pH 6.0 | Saccharomyces cerevisiae | |
0.0188 | - |
Fe(II) | mutant D409A, pH 6.0 | Saccharomyces cerevisiae | |
0.0193 | - |
Fe(II) | mutant D283A, pH 6.0 | Saccharomyces cerevisiae | |
0.0356 | - |
Fe(II) | mutant E185A, pH 6.0 | Saccharomyces cerevisiae | |
3.994 | - |
Fe(II) | mutant E185A/D409A, pH 6.0 | Saccharomyces cerevisiae | |
18.2 | - |
hydroquinone | mutant E185A, pH 6.0 | Saccharomyces cerevisiae | |
19.3 | - |
hydroquinone | mutant D283A, pH 6.0 | Saccharomyces cerevisiae | |
25.5 | - |
hydroquinone | wild-type, pH 6.0 | Saccharomyces cerevisiae | |
30.3 | - |
hydroquinone | mutant D409A, pH 6.0 | Saccharomyces cerevisiae | |
30.5 | - |
hydroquinone | mutant E185A/D409A, pH 6.0 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 Fe(II) + 4 H+ + O2 = 4 Fe(III) + 2 H2O | residues D283, E185, D409 provide a Fe(II) binding site that favors ferric ion thus reducing the reduction potential of the bound Fe(II). Residues E185 and D409 form part of the electron-transfer pathway from the bound Fe(II) to the proteins type I Cu(II) | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(II) + H+ + O2 | - |
Saccharomyces cerevisiae | Fe(III) + H2O | - |
? | |
Fe(II) + hydroquinone + O2 | - |
Saccharomyces cerevisiae | Fe(III) + ? + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.232 | - |
Fe(II) | mutant E185A/D409A, pH 6.0 | Saccharomyces cerevisiae | |
0.405 | - |
Fe(II) | mutant E185A, pH 6.0 | Saccharomyces cerevisiae | |
0.66 | - |
Fe(II) | wild-type, pH 6.0 | Saccharomyces cerevisiae | |
0.805 | - |
Fe(II) | mutant D409A, pH 6.0 | Saccharomyces cerevisiae | |
1.26 | - |
Fe(II) | mutant D283A, pH 6.0 | Saccharomyces cerevisiae | |
1.53 | - |
hydroquinone | mutant E185A, pH 6.0 | Saccharomyces cerevisiae | |
2.2 | - |
hydroquinone | wild-type, pH 6.0 | Saccharomyces cerevisiae | |
2.3 | - |
hydroquinone | mutant E185A/D409A, pH 6.0 | Saccharomyces cerevisiae | |
2.33 | - |
hydroquinone | mutant D409A, pH 6.0 | Saccharomyces cerevisiae | |
2.81 | - |
hydroquinone | mutant D283A, pH 6.0 | Saccharomyces cerevisiae | |
6.08 | - |
Fe(II) | mutant D409A, pH 6.0 | Saccharomyces cerevisiae |