Activating Compound | Comment | Organism | Structure |
---|---|---|---|
flavin | the enzyme is able to reduce iron compounds in the absence of free flavin, but the ferric reduction by the enzyme is enhanced by the addition of free flavin n the presence of natural chelate iron compounds but also synthetic chelate iron compounds | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0168 | - |
Fe(III)-EDTA | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
0.0276 | - |
Fe(III)-citrate | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
0.16 | - |
Fe(III)-citrate | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
0.5762 | - |
Fe(III)-EDTA | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Synechocystis sp. |
60000 | - |
gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II)-siderophore + NADP+ + H+ | Synechocystis sp. | - |
2 Fe(III)-siderophore + NADPH | - |
? | |
additional information | Synechocystis sp. | DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide is potently enhanced by the purified enzyme, indicating that the enzyme may drive the Fenton reaction, reducing ferric iron to ferrous iron when it evokes the Fenton reaction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl Toyopearl column chromatography, DEAE Sepharose column chromatography, POROS HQ-H column chromatography, and Red Sepharose column chromatography | Synechocystis sp. |
native enzyme | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
crude extract, using tert-butyl hydroperoxide as substrate, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. |
0.91 | - |
after 45.5fold purification, using tert-butyl hydroperoxide as substrate, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II) + NADP+ + H+ | - |
Synechocystis sp. | 2 Fe(III) + NADPH | - |
? | |
2 Fe(II)-cytochrome c + NADP+ + H+ | - |
Synechocystis sp. | 2 Fe(III)-cytochrome c + NADPH | - |
? | |
2 Fe(II)-siderophore + NADP+ + H+ | - |
Synechocystis sp. | 2 Fe(III)-siderophore + NADPH | - |
? | |
2 ferricyanide + NADPH | - |
Synechocystis sp. | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferrocyanide + NADP+ + H+ | - |
Synechocystis sp. | 2 ferricyanide + NADPH | - |
? | |
2,6-dichloroindophenol + NADPH + H+ | best substrate in the presence and absence of FAD | Synechocystis sp. | ? | - |
? | |
cytochrome c + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
Fe(III)-citrate + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
Fe(III)-deferoxamine + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
Fe(III)-diethylenetriamine-N,N,N',N'',N''-pentaacetate + NADPH + H+ | - |
Synechocystis sp. | Fe(II) + diethylenetriamine-N,N,N',N'',N''-pentaacetate + NADP+ + H+ | - |
? | |
Fe(III)-EDTA + NADPH + H+ | - |
Synechocystis sp. | Fe(II) + EDTA + NADP+ + H+ | - |
? | |
Fe(III)-ferrichrome + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
Fe(III)-nitrilotriacetic acid + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
ferric ammonium citrate + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
ferritin + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
additional information | no activity with NADH. In the absence of FAD, the enzyme shows no activity towards ferric citrate, Fe(III)-ferrichrome, Fe(III)-deferoxamine, Fe(III)-nitrilotriacetic acid, and transferrin | Synechocystis sp. | ? | - |
? | |
additional information | DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide is potently enhanced by the purified enzyme, indicating that the enzyme may drive the Fenton reaction, reducing ferric iron to ferrous iron when it evokes the Fenton reaction | Synechocystis sp. | ? | - |
? | |
additional information | the purified enzyme reacts with cytochrome c, ferricyanide and 2,6-dichloroindophenol, the flavin-independent NADPH oxidoreductase elicites NADPH oxidation activity during reduction of t-butyl hydroperoxide in the presence of Fe(III)-EDTA | Synechocystis sp. | ? | - |
? | |
tert-butyl hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? | |
transferrin + NADPH + H+ | - |
Synechocystis sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000, SDS-PAGE | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP(+) oxidoreductase | - |
Synechocystis sp. |
ferredoxin-NADP+ oxidoreductase | - |
Synechocystis sp. |
FNR(S) | - |
Synechocystis sp. |
FNRS | - |
Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
Fe(III)-citrate | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
0.95 | - |
Fe(III)-EDTA | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
1.11 | - |
Fe(III)-citrate | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
2 | - |
Fe(III)-EDTA | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is flavin-independent | Synechocystis sp. | |
NADPH | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is driving the Fenton reaction | Synechocystis sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
Fe(III)-EDTA | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
1.59 | - |
Fe(III)-citrate | in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
41 | - |
Fe(III)-citrate | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. | |
122 | - |
Fe(III)-EDTA | in the presence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C | Synechocystis sp. |