Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of truncation mutant S698DELTA, which shows increased activity with cytochrome c3+ as substrate compared to the wild-type enzyme | Homo sapiens |
S698A | site-directed mutagenesis, the mutant shows reduced activity with cytochrome c3+ as substrate compared to the wild-type enzyme, the S698A mutant displays a 6fold reduction in kcat/Km for NADPH | Homo sapiens |
W697H | site-directed mutagenesis, the mutant shows increased activity with cytochrome c3+ as substrate compared to the wild-type enzyme | Homo sapiens |
W697S | site-directed mutagenesis, the mutant shows reduced activity with cytochrome c3+ as substrate compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-ADP | competitive inhibition | Homo sapiens | |
NADP+ | competitive inhibition in the reaction with NADPH and cytochrome c3+ as substrates, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics analysis of wild-type and mutant enzymes with cytochrome c3+ and NADPH as substrates, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ | Homo sapiens | - |
2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBK8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ | - |
Homo sapiens | 2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine | - |
? | |
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ | the enzyme catalyzes the oxidation of NADPH and shuttles electrons via its FAD and FMN cofactors to inactive MScob(II)alamin | Homo sapiens | 2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine | - |
? | |
additional information | the enzyme catalyzes the reduction of cytochrome c3+ with NADPH and NADH | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Methionine synthase reductase | - |
Homo sapiens |
MSR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme is a diflavin oxidoreductase, binding structure, overview | Homo sapiens | |
FMN | the enzyme is a diflavin oxidoreductase, binding structure, overview | Homo sapiens | |
NADP+ | binding structure, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | methionine synthase reductase, a diflavin oxidoreductase, plays a vital role in methionine and folate metabolism by sustaining methionine synthase activity | Homo sapiens |