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Literature summary for 1.16.1.8 extracted from

  • Meints, C.E.; Gustafsson, F.S.; Scrutton, N.S.; Wolthers, K.R.
    Tryptophan 697 modulates hydride and interflavin electron transfer in human methionine synthase reductase (2011), Biochemistry, 50, 11131-11142.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information generation of truncation mutant S698DELTA, which shows increased activity with cytochrome c3+ as substrate compared to the wild-type enzyme Homo sapiens
S698A site-directed mutagenesis, the mutant shows reduced activity with cytochrome c3+ as substrate compared to the wild-type enzyme, the S698A mutant displays a 6fold reduction in kcat/Km for NADPH Homo sapiens
W697H site-directed mutagenesis, the mutant shows increased activity with cytochrome c3+ as substrate compared to the wild-type enzyme Homo sapiens
W697S site-directed mutagenesis, the mutant shows reduced activity with cytochrome c3+ as substrate compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2',5'-ADP competitive inhibition Homo sapiens
NADP+ competitive inhibition in the reaction with NADPH and cytochrome c3+ as substrates, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics analysis of wild-type and mutant enzymes with cytochrome c3+ and NADPH as substrates, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ Homo sapiens
-
2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBK8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+
-
Homo sapiens 2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine
-
?
2 [methionine synthase]-methylcob(I)alamin + 2 S-adenosylhomocysteine + NADP+ the enzyme catalyzes the oxidation of NADPH and shuttles electrons via its FAD and FMN cofactors to inactive MScob(II)alamin Homo sapiens 2 [methionine synthase]-cob(II)alamin + NADPH + H+ + 2 S-adenosyl-L-methionine
-
?
additional information the enzyme catalyzes the reduction of cytochrome c3+ with NADPH and NADH Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Methionine synthase reductase
-
Homo sapiens
MSR
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme is a diflavin oxidoreductase, binding structure, overview Homo sapiens
FMN the enzyme is a diflavin oxidoreductase, binding structure, overview Homo sapiens
NADP+ binding structure, overview Homo sapiens

General Information

General Information Comment Organism
physiological function methionine synthase reductase, a diflavin oxidoreductase, plays a vital role in methionine and folate metabolism by sustaining methionine synthase activity Homo sapiens