Crystallization (Comment) | Organism |
---|---|
MSR NADP(H)/FAD domain complex, sitting drop vapor diffusion method, 4°C, 10 mg/ml protein in 10 mM Tris/HCl, pH 8.0, 0.5 mM DTT, 1 mM EDTA, and 0.05% NaN3, reservoir solution comprising 0.1 M Tris/HCl, pH 7.5, 0.2 M KBr, and 15% PEG 4000, crystal soaking in saturated NADP+ solution, X-ray diffraction structure determination and analysis at 1.9 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-ADP | inhibition of MSR-catalyzed reduction of cytochrome c3+ | Homo sapiens | |
NADP+ | inhibition of MSR-catalyzed reduction of cytochrome c3+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | isothermal titration calorimetry reveals a binding constant of 0.037 and 0.002 mM for binding of NADP+ and 2',5'-ADP, respectively, for the ligand-protein complex formed with full-length MSR or the isolated FNR module | Homo sapiens | |
0.00289 | - |
NADPH | pH 7.5, 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | biological implications of an attenuated mechanism of MS reactivation by MSR on methionine and folate metabolism, overview | ? | - |
? | |
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | Homo sapiens | - |
[methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | biological implications of an attenuated mechanism of MS reactivation by MSR on methionine and folate metabolism, overview | Homo sapiens | ? | - |
? | |
additional information | the enzyme catalyzes also the inhibition of reduction of cytochrome c3+ | Homo sapiens | ? | - |
? | |
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | - |
Homo sapiens | [methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r | |
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | MSR is a flavoprotein that regenerates the active form of cobalamin-dependent methionine synthase | Homo sapiens | [methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Homo sapiens |
More | the NADP+-bound FNR-like module of MSR spans the NADP(H)-binding domain, the FAD-binding domain, the connecting domain, and part of the extended hinge region | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Methionine synthase reductase | - |
Homo sapiens |
MSR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.92 | - |
NADPH | pH 7.5, 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | MSR contains one FAD and one FMN cofactor per polypeptide and functions in the sequential transfer of reducing equivalents from NADPH to MS via its flavin centers | Homo sapiens | |
FMN | MSR contains one FAD and one FMN cofactor per polypeptide and functions in the sequential transfer of reducing equivalents from NADPH to MS via its flavin centers | Homo sapiens | |
additional information | NAD(H) is a poor cofactor | Homo sapiens | |
NADP+ | dependent on, binding structure, overview, the NADP+-bound FNR-like module of MSR spans the NADP(H)-binding domain, the FAD-binding domain, the connecting domain, and part of the extended hinge region | Homo sapiens | |
NADPH | dependent on, binding structure, overview, the NADP+-bound FNR-like module of MSR spans the NADP(H)-binding domain, the FAD-binding domain, the connecting domain, and part of the extended hinge region | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state inhibition studies | Homo sapiens | |
0.0014 | - |
2',5'-ADP | inhibition of reduction of cytochrome c3+ | Homo sapiens | |
0.036 | - |
NADP+ | inhibition of reduction of cytochrome c3+ | Homo sapiens |