Cloned (Comment) | Organism |
---|---|
gene LeFRO1, DNA and amino acid sequence determination and analysis from 16 different cultivars, genotyping detects three types of LeFRO1 (LeFRO1MM, LeFRO1Ailsa and LeFRO1Monita) with five amino acid variations at the positions 21, 24, 112, 195 and 582 | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
A112X | modification of this amino acid results in a significant reduction of enzyme activity | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II)-siderophore + NAD+ + H+ | Solanum lycopersicum | - |
2 Fe(III)-siderophore + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
16 cultivars | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Fe(II)-siderophore + NAD+ + H+ | - |
Solanum lycopersicum | 2 Fe(III)-siderophore + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ferric-chelate reductase | - |
Solanum lycopersicum |
FRO1 | - |
Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
additional information | Ala112 of LeFRO1 is critical for maintaining the high activity of ferric-chelate reductase, modification of this amino acid results in a significant reduction of enzyme activity. The combination of the amino acid residue Ile at the site 24 with Lys at the site 582 plays a positive role in the enzyme activity of LeFRO1, genotyping, overview | Solanum lycopersicum |
physiological function | ferric-chelate reductase which functions in the reduction of ferric to ferrous iron on root surface is a critical protein for iron homeostasis in strategy I plants. LeFRO1 is a major ferric-chelate reductase involved in iron uptake in tomato | Solanum lycopersicum |