Literature summary for 1.16.1.10 extracted from

Chiu, H.J.; Johnson, E.; Schroeder, I.; Rees, D.C.
Crystal structures of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus and its complex with NADP+ (2001), Structure, 9, 311-319.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of the homodimeric enzyme are grown at 4°C by the hanging drop vapor diffusion technique, with protein solution at a concentration of 25 mg/ml in 0.1 M Tris-HCl (pH 7.5) and a reservoir consisting of 21%22% w/v PEG4000, 0.1 M sodium acetate, and 0.1 M Tris-HCl (pH 8.4). The crystallization drops are microseeded after 2024 hr. Crystal structure of recombinant enzyme containing a bound FMN solved at 1.5 A resolution. The NADP+-bound enzyme complex is determined at 1.65 A resolution	Archaeoglobus fulgidus

Crystallization (Comment)

Organism

crystals of the homodimeric enzyme are grown at 4°C by the hanging drop vapor diffusion technique, with protein solution at a concentration of 25 mg/ml in 0.1 M Tris-HCl (pH 7.5) and a reservoir consisting of 21%22% w/v PEG4000, 0.1 M sodium acetate, and 0.1 M Tris-HCl (pH 8.4). The crystallization drops are microseeded after 2024 hr. Crystal structure of recombinant enzyme containing a bound FMN solved at 1.5 A resolution. The NADP+-bound enzyme complex is determined at 1.65 A resolution

Archaeoglobus fulgidus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	high cellular abundance, 0.75% of the total soluble protein	Archaeoglobus fulgidus	-	-

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O29428	-	-

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Release 2023.1 (January 2023)