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Literature summary for 1.16.1.1 extracted from
- The isolation of a mercuric ion-reducing flavoprotein from Thiobacillus ferrooxidans (1984), J. Gen. Microbiol., 130, 725-730.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | AgNO3 | Acidithiobacillus ferrooxidans |  |
| CoCl2 | CoCl2 | Acidithiobacillus ferrooxidans | |
| Cu2+ | CuCl2 | Acidithiobacillus ferrooxidans | |
| CuCl | - |
Acidithiobacillus ferrooxidans | |
| FeCl3 | - |
Acidithiobacillus ferrooxidans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0089 | - |
Hg | - |
Acidithiobacillus ferrooxidans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000 + x * 62000, SDS-PAGE | Acidithiobacillus ferrooxidans |
| 62000 | - |
x * 54000 + x * 62000, SDS-PAGE | Acidithiobacillus ferrooxidans |
| 130000 | - |
gel filtration | Acidithiobacillus ferrooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | - |
TFI 29 | - |
| Acidithiobacillus ferrooxidans TFI 29 | - |
TFI 29 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidithiobacillus ferrooxidans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.8 | - |
- |
Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hg + NADP+ + H+ | - |
Acidithiobacillus ferrooxidans | Hg2+ + NADPH | - |
r | |
| Hg + NADP+ + H+ | - |
Acidithiobacillus ferrooxidans TFI 29 | Hg2+ + NADPH | - |
r | |
| Hg2+ + NADPH | - |
Acidithiobacillus ferrooxidans | Hg + NADP+ + H+ | - |
? | |
| Hg2+ + NADPH | - |
Acidithiobacillus ferrooxidans TFI 29 | Hg + NADP+ + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x * 54000 + x * 62000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.4 | - |
HgCl2 | - |
Acidithiobacillus ferrooxidans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acidithiobacillus ferrooxidans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Acidithiobacillus ferrooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains FAD | Acidithiobacillus ferrooxidans | |
| NADPH | - |
Acidithiobacillus ferrooxidans | |
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