Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21-Gold (DE3) | Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | one Fe per monomer, consistent with full occupancy of the metal center in the active site of the enzyme | Giardia intestinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + 2 H+ | Giardia intestinalis | - |
rubredoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + 2 H+ | Giardia intestinalis WB clone C6 | - |
rubredoxin + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Giardia intestinalis WB clone C6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, gel filtration and cleavage of the His-tag, followed by a second time nickel affinity chromatography and gel filtration | Giardia intestinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Giardia intestinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + 2 H+ | - |
Giardia intestinalis | rubredoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + 2 H+ | - |
Giardia intestinalis WB clone C6 | rubredoxin + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Giardia intestinalis |
Synonyms | Comment | Organism |
---|---|---|
SOR | - |
Giardia intestinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Giardia intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Giardia intestinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
rubredoxin | rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs | Giardia intestinalis |
General Information | Comment | Organism |
---|---|---|
evolution | Giardia trophozoite expresses an enzyme probably acquired from a prokaryote by lateral gene transfer. Rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs | Giardia intestinalis |
additional information | pH dependent ligand exchange in the final intermediate, overview | Giardia intestinalis |
physiological function | Giardia trophozoite expresses a SOR possibly involved in superoxide detoxification | Giardia intestinalis |