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Literature summary for 1.15.1.2 extracted from

  • Testa, F.; Mastronicola, D.; Cabelli, D.E.; Bordi, E.; Pucillo, L.P.; Sarti, P.; Saraiva, L.M.; Giuffre, A.; Teixeira, M.
    The superoxide reductase from the early diverging eukaryote Giardia intestinalis (2011), Free Radic. Biol. Med., 51, 1567-1574.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21-Gold (DE3) Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ one Fe per monomer, consistent with full occupancy of the metal center in the active site of the enzyme Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced rubredoxin + superoxide + 2 H+ Giardia intestinalis
-
rubredoxin + H2O2
-
?
reduced rubredoxin + superoxide + 2 H+ Giardia intestinalis WB clone C6
-
rubredoxin + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Giardia intestinalis WB clone C6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, gel filtration and cleavage of the His-tag, followed by a second time nickel affinity chromatography and gel filtration Giardia intestinalis

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Giardia intestinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced rubredoxin + superoxide + 2 H+
-
Giardia intestinalis rubredoxin + H2O2
-
?
reduced rubredoxin + superoxide + 2 H+
-
Giardia intestinalis WB clone C6 rubredoxin + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer
-
Giardia intestinalis

Synonyms

Synonyms Comment Organism
SOR
-
Giardia intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
assay at Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
rubredoxin rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs Giardia intestinalis

General Information

General Information Comment Organism
evolution Giardia trophozoite expresses an enzyme probably acquired from a prokaryote by lateral gene transfer. Rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs Giardia intestinalis
additional information pH dependent ligand exchange in the final intermediate, overview Giardia intestinalis
physiological function Giardia trophozoite expresses a SOR possibly involved in superoxide detoxification Giardia intestinalis