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Literature summary for 1.15.1.2 extracted from

  • Caranto, J.D.; Gebhardt, L.L.; MacGowan, C.E.; Limberger, R.J.; Kurtz, D.M.
    Treponema denticola superoxide reductase: in vivo role, in vitro reactivities, and a novel [Fe(Cys)(4)] site (2012), Biochemistry, 51, 5601-5610.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.15.1.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Treponema denticola

Protein Variants

Protein Variants Comment Organism
additional information construction of inactive 1754M strain containing the insertionally inactivated Td SOR gene Treponema denticola

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ non-heme [Fe(His)4Cys] active sites Treponema denticola
additional information no other metal-binding domain besides the non-heme [Fe(His)4Cys] sites Treponema denticola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced rubredoxin + superoxide + 2 H+ Treponema denticola
-
 rubredoxin + H2O2
-
 ?
reduced rubredoxin + superoxide + 2 H+ Treponema denticola 35405
-
 rubredoxin + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
-
-
Treponema denticola 35405
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange and metal-chelate affinity chromatography Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced rubredoxin + superoxide + 2 H+
-
 Treponema denticola rubredoxin + H2O2
-
 ?
reduced rubredoxin + superoxide + 2 H+ with NADH Treponema denticola rubredoxin + H2O2
-
 ?
reduced rubredoxin + superoxide + 2 H+
-
 Treponema denticola 35405 rubredoxin + H2O2
-
 ?
reduced rubredoxin + superoxide + 2 H+ with NADH Treponema denticola 35405 rubredoxin + H2O2
-
 ?

Subunits

Subunits Comment Organism
More homology structural modeling using the Tp SOR structure, PDB ID 1Y07, as template, overview Treponema denticola

Synonyms

Synonyms Comment Organism
SOR
-
 Treponema denticola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Treponema denticola

Cofactor

Cofactor Comment Organism Structure
rubredoxin contains on-heme [Fe(His)4Cys] active sites. Enzymatic reduction of the enzyme iron sites with use of NADH:rubredoxin oxidoreductase and rubredoxin, both from Thermotoga maritima, rates of enzymatic reduction of the ferric center I and enzyme sites are measured Treponema denticola

General Information

General Information Comment Organism
malfunction the enzyme-inactivated 1754M strain is significantly more air-sensitive than the wild-type strain on NOS agarose plates exposed to air Treponema denticola
additional information the enzyme contains non-heme [Fe(His)4Cys] active sites, homology structural modeling using the Tp SOR structure, PDB ID 1Y07, as template, overview Treponema denticola
physiological function superoxide reductase from the air-sensitive oral spirochete Treponema denticola is a principal enzymatic scavenger of superoxide in this organism, role for the enzyme in oxidative stress protection of O2-exposed Treponema denticola 35405 Treponema denticola