Protein Variants | Comment | Organism |
---|---|---|
C13S | mutant enzyme kinetics in comparison to the wild-type enzyme | Desulfovibrio vulgaris |
E47A | the mutation of 2Fe-SOR results in an identical 600 nm intermediate that decays at the same rate as for the wild-type protein at and above neutral pH, but to a solvent- rather than glutamate-ligated resting ferric SOR site, mutant enzyme kinetics in comparison to the wild-type enzyme | Desulfovibrio vulgaris |
K48A | mutant enzyme kinetics in comparison to the wild-type enzyme | Desulfovibrio vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Desulfovibrio vulgaris | |
fluoride | - |
Desulfovibrio vulgaris | |
formate | - |
Desulfovibrio vulgaris | |
phosphate | - |
Desulfovibrio vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | solvent deuterium isotope effects, stopped-flow kinetics of wild-type and mutant enzymes at different pH in different buffer systems, overview | Desulfovibrio vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | two-iron superoxide reductase with a ferrous active site | Desulfovibrio vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + H+ | Desulfovibrio vulgaris | - |
rubredoxin + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced rubredoxin + superoxide + H+ | - |
Desulfovibrio vulgaris | rubredoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + H+ | probably via a ferric-hydroperoxo intermediate, which decays smoothly to the resting ferric active site with no other detectable intermediates, solvent proton donation occurs in the rate-determining step of dead time intermediate decay and neither of the conserved pocket residues, Glu47 or Lys48, functions as a rate-determining proton donor between pH 6.0 and pH 8.0 | Desulfovibrio vulgaris | rubredoxin + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2Fe-SOR | - |
Desulfovibrio vulgaris |
two-iron superoxide reductase | - |
Desulfovibrio vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Desulfovibrio vulgaris |