Literature summary for 1.15.1.1 extracted from

Zhu, Y.; Wang, G.; Ni, H.; Xiao, A.; Cai, H.
Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 (2014), World J. Microbiol. Biotechnol., 30, 1347-1357 .

Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Geobacillus sp. EPT3

Inhibitors

Inhibitors	Comment	Organism	Structure
Ba2+	inhibits at 1 mM	Geobacillus sp. EPT3
Ca2+	inhibits at 1 mM	Geobacillus sp. EPT3
Co2+	inhibits at 1 mM	Geobacillus sp. EPT3
Cu2+	inhibits at 1 mM	Geobacillus sp. EPT3
Fe2+	inhibits at 1 mM	Geobacillus sp. EPT3
Mg2+	inhibits at 1 mM	Geobacillus sp. EPT3
additional information	insensitivity of the recombinant enzyme to both KCN and H2O2. The enzyme shows a relatively good tolerance to some inhibitors, detergents, and denaturants, such as 2-mercaptoethanol, dithiothreitol, phenylmethylsulfonyl fluoride, Chaps, Triton X-100, urea, and guanidine hydrochloride	Geobacillus sp. EPT3
Ni2+	inhibits slightly at 1 mM	Geobacillus sp. EPT3
Zn2+	inhibits at 1 mM	Geobacillus sp. EPT3

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	a Mn-SOD, Mn2+ activate the enzyme at 1 mM, the manganese-binding sites are conserved in the sequence involving residues His260, His308, Asp392, and His396	Geobacillus sp. EPT3
additional information	the Fe2+-reconstituted SOD is inactive	Geobacillus sp. EPT3

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130700	-	recombinant His-tagged enzyme, gel filtration	Geobacillus sp. EPT3

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 superoxide + 2 H+	Geobacillus sp. EPT3	-	O2 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp. EPT3	T1T1K2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Geobacillus sp. EPT3

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1204	-	purified recombinant His-tagged enzyme, pH 7.8, 25°C	Geobacillus sp. EPT3

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 superoxide + 2 H+	-	Geobacillus sp. EPT3	O2 + H2O2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 59000, recombinant His-tagged enzyme, SDS-PAGE	Geobacillus sp. EPT3

Synonyms

Synonyms	Comment	Organism
manganese-containing SOD	-	Geobacillus sp. EPT3

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Geobacillus sp. EPT3

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	purified recombinant enzyme, 1 h, stable at	Geobacillus sp. EPT3
90	-	purified recombinant enzyme, 57% activity remaining after incubation for 1 h	Geobacillus sp. EPT3

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Geobacillus sp. EPT3

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	11	purified recombinant enzyme, 1 h, highly stable at	Geobacillus sp. EPT3

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Release 2023.1 (January 2023)