Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli | Curcuma aromatica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 17% inhibition at 3 mM | Curcuma aromatica | |
DTT | 13% inhibition at 2.5 mM | Curcuma aromatica | |
EDTA | 15% inhibition at 0.5 mM | Curcuma aromatica | |
guanidinium hydrochloride | 40% inhibition at 2 M, 85% at 5 M | Curcuma aromatica | |
H2O2 | 58% inhibition at 2 mM | Curcuma aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MichaelisMenten and LineweaverBurk kinetics | Curcuma aromatica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu,Zn SOD | Curcuma aromatica | |
Zn2+ | a Cu,Zn SOD | Curcuma aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Curcuma aromatica | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curcuma aromatica | E6Y8N8 | collected at 10:00 am from the experimental farm of the Institute of Himalayan Bioresource Technology at Palampur, Distt. Kangra, India | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis | Curcuma aromatica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Curcuma aromatica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1250 | - |
purified recombinant His-tagged enzyme, pH 7.8, 37°C, substrate riboflavin | Curcuma aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Curcuma aromatica | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 17500, about, sequence calculation, 2 * 18800, about, SDS-PAGE | Curcuma aromatica |
Synonyms | Comment | Organism |
---|---|---|
Ca-Cu,Zn SOD | - |
Curcuma aromatica |
Cu,Zn superoxide dismutase | - |
Curcuma aromatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Curcuma aromatica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
80 | activity range between -10-80°C | Curcuma aromatica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70.45 | - |
midpoint of thermal transition (Tm) | Curcuma aromatica |
121 | - |
Ca-Cu Z- SOD retains 50% of the maximum activity after autoclaving, autoclaved Ca-Cu,Zn SOD exhibits 40% of maximum activity of unautoclaved enzyme at 20°C and 30°C. Thermal inactivation kinetics of Ca-Cu,Zn SOD, overview | Curcuma aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Curcuma aromatica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range | Curcuma aromatica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | CaCu,Zn SOD is stable at a wide storage pH ranging from pH 3 to pH 10. After24 h of incubation, the enzyme retains more than 80% of maximal activity in the pH range of pH 3.0-9.0, whereas at pH 10.0 the enzyme loses 36.21% of maximal activity, profile overview | Curcuma aromatica |