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Literature summary for 1.15.1.1 extracted from
- Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus x giganteus (2015), Plant Cell Rep., 34, 2137-2149 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MgMnSOD1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, tissue expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3). Expression of HBT95-MgMnSOD1-sGFP vector driven by a CaMV 35S promoter to determine the intracellular localization of MgMnSOD1 | Miscanthus x giganteus |
| gene MgMnSOD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, tissue expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3) | Miscanthus x giganteus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | surrounding the central vacuole | Miscanthus x giganteus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | a MgMnSOD | Miscanthus x giganteus |  |
| Mn2+ | a MgMnSOD | Miscanthus x giganteus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | Miscanthus x giganteus | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Miscanthus x giganteus | A0A088MAG2 | - |
- |
| Miscanthus x giganteus | A0A088MM36 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography | Miscanthus x giganteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| internode | - |
Miscanthus x giganteus | - |
| leaf | - |
Miscanthus x giganteus | - |
| leaf bud | - |
Miscanthus x giganteus | - |
| leaf sheath | - |
Miscanthus x giganteus | - |
| additional information | organs from eight-leaf growth stage, enzyme MgMnSOD1 expression pattern, overview | Miscanthus x giganteus | - |
| additional information | organs from eight-leaf growth stage, enzyme MgMnSOD2 expression pattern, overview | Miscanthus x giganteus | - |
| node | - |
Miscanthus x giganteus | - |
| rhizome | - |
Miscanthus x giganteus | - |
| root | - |
Miscanthus x giganteus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1854.79 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Miscanthus x giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | - |
Miscanthus x giganteus | O2 + H2O2 | - |
? | |
| additional information | enzyme activity of recombinant MgMnSOD1 is assayed by using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, i.e. WST-1. The method shows high sensitivity due to the lower reaction rate of WST-1 and superoxide anion | Miscanthus x giganteus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, recombinant His-tagged enzyme, SDS-PAGE, x * 30960, recombinant His-tagged enzyme, sequence calculation | Miscanthus x giganteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| manganese superoxide dismutase | - |
Miscanthus x giganteus |
| MgMnSOD1 | - |
Miscanthus x giganteus |
| MgMnSOD2 | - |
Miscanthus x giganteus |
| MnSOD1 | - |
Miscanthus x giganteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | purified recombinant His-tagged enzyme, more than 73% activity remains after 30 min. The residual activity declines sharply from 73.07% to 28.75% between 60°C and 70°C, T1/2 is 63°C | Miscanthus x giganteus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | sequence analysis and expression patterns of MgMnSOD1 and MgMnSOD2 suggest that they are orthologous genes which are inherited from the two parents, Miscanthus sacchariflora and Miscanthus sinensis, respectively | Miscanthus x giganteus |
| physiological function | manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme involved in stress tolerance and able to protect plant cells from accumulated reactive oxygen species by converting superoxide to peroxide and oxygen | Miscanthus x giganteus |
| physiological function | manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme involved in stress tolerance and able to protect plant cells from accumulated reactive oxygen species by converting superoxide to peroxide and oxygen. Enzyme MgMnSOD1 is predicted to be targeted to mitochondria and involved in removing the superoxide radical generated by respiration | Miscanthus x giganteus |
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