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Literature summary for 1.15.1.1 extracted from

  • Kan, G.; Wen, H.; Wang, X.; Zhou, T.; Shi, C.
    Cloning and characterization of iron-superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5 (2017), J. Basic Microbiol., 57, 680-690 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene RmFeSOD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3). Under copper stress experiment, the RmFeSOD-expressing Escherichia coli cells exhibit a better growth than nontransformed bacteria Rhodotorula mucilaginosa

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Rhodotorula mucilaginosa
DMSO
-
Rhodotorula mucilaginosa
Mg2+ 30% inhibition at 1 mM Rhodotorula mucilaginosa
additional information no inhibition by DTT. Ba2+, Ca2+, Ni2+, and Fe2+ have no obvious impact to enzyme activity at 1 mM Rhodotorula mucilaginosa
PMSF
-
Rhodotorula mucilaginosa
SDS 70% inhibition Rhodotorula mucilaginosa
Triton X-100 70% inhibition Rhodotorula mucilaginosa
Tween-80 70% inhibition Rhodotorula mucilaginosa
Urea
-
Rhodotorula mucilaginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
Fe2+ required, a Fe-SOD Rhodotorula mucilaginosa
Fe3+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
Mn2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa
Zn2+ activates the enzyme at 1 mM Rhodotorula mucilaginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49300
-
recombinant His-tagged enzyme, gel filtration Rhodotorula mucilaginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Rhodotorula mucilaginosa
-
O2 + H2O2
-
?
2 superoxide + 2 H+ Rhodotorula mucilaginosa AN5
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rhodotorula mucilaginosa A0A1L5JYH9
-
-
Rhodotorula mucilaginosa AN5 A0A1L5JYH9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Rhodotorula mucilaginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
Rhodotorula mucilaginosa O2 + H2O2
-
?
2 superoxide + 2 H+
-
Rhodotorula mucilaginosa AN5 O2 + H2O2
-
?
additional information enzyme activity measurement by reduction of NBT Rhodotorula mucilaginosa ?
-
?
additional information enzyme activity measurement by reduction of NBT Rhodotorula mucilaginosa AN5 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 23500, about, sequence calculation, 2 * 31000, recombinant His-tagged enzyme, SDS-PAGE Rhodotorula mucilaginosa

Synonyms

Synonyms Comment Organism
iron SOD type
-
Rhodotorula mucilaginosa
RmFeSOD
-
Rhodotorula mucilaginosa
SOD
-
Rhodotorula mucilaginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rhodotorula mucilaginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 recombinant enzyme, 1 h, about 90% activity remaining, completely stable for 45 min Rhodotorula mucilaginosa
50
-
recombinant enzyme, 1 h, 80% activity remaining Rhodotorula mucilaginosa
60
-
recombinant enzyme, 1 h, 60% activity remaining Rhodotorula mucilaginosa
70
-
recombinant enzyme, 1 h, 20% activity remaining Rhodotorula mucilaginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4
-
Rhodotorula mucilaginosa

pH Range

pH Minimum pH Maximum Comment Organism
1 11 activity range, 75% of maximal activity at pH 1.0, 45% at pH 11.0, profile overview Rhodotorula mucilaginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 9 recombinant RmFeSOD shows good pH stability in the pH 1.0-9.0 after 1 h incubation Rhodotorula mucilaginosa

pI Value

Organism Comment pI Value Maximum pI Value
Rhodotorula mucilaginosa sequence calculation
-
7.89

General Information

General Information Comment Organism
additional information the conserved histidine residues (His41, His89, His179), aspartate residue (Asp175), and WEHAYY domain play an important role in the active site of the Fe-binding sites Rhodotorula mucilaginosa
physiological function enzyme RmFeSOD plays an important role in the adaptability of heavy metals Rhodotorula mucilaginosa