Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.15.1.1 extracted from

  • Wang, Q.F.; Wang, Y.F.; Hou, Y.H.; Shi, Y.L.; Han, H.; Miao, M.; Wu, Y.Y.; Liu, Y.P.; Yue, X.N.; Li, Y.J.
    Cloning, expression and biochemical characterization of recombinant superoxide dismutase from Antarctic psychrophilic bacterium Pseudoalteromonas sp. ANT506 (2016), J. Basic Microbiol., 56, 753-761 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudoalteromonas sp. ANT506

Inhibitors

Inhibitors Comment Organism Structure
chloroform recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture Pseudoalteromonas sp. ANT506
ethanol recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture Pseudoalteromonas sp. ANT506
Fe3+ 9.7% inhibition Pseudoalteromonas sp. ANT506
H2O2 preincubation in 5 mM H2O2 for 30 min results in a loss of 91% activity Pseudoalteromonas sp. ANT506
NaCl 60% activity remains at 2.5 M NaCl Pseudoalteromonas sp. ANT506
Ni2+ 33.7% inhibition Pseudoalteromonas sp. ANT506

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Pseudoalteromonas sp. ANT506
Cu2+ activates Pseudoalteromonas sp. ANT506
Fe2+ a Fe/Mn-SOD Pseudoalteromonas sp. ANT506
Mg2+ activates Pseudoalteromonas sp. ANT506
Mn2+ a Fe/Mn-SOD Pseudoalteromonas sp. ANT506
Zn2+ activates Pseudoalteromonas sp. ANT506

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Pseudoalteromonas sp. ANT506
-
O2 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. ANT506 A0A0S2E8T0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 12.6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudoalteromonas sp. ANT506

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
Pseudoalteromonas sp. ANT506 O2 + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 21400, about, sequence calculation, 1 * 27000, recombinant His-tagged enzyme, SDS-PAGE Pseudoalteromonas sp. ANT506

Synonyms

Synonyms Comment Organism
Fe/Mn-SOD
-
Pseudoalteromonas sp. ANT506
PsSOD
-
Pseudoalteromonas sp. ANT506
SOD
-
Pseudoalteromonas sp. ANT506

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant His-tagged enzyme Pseudoalteromonas sp. ANT506

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
50 activity range, 13.9% of maximal activity at 0°C, 25.7% at 10°C Pseudoalteromonas sp. ANT506

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant His-tagged enzyme, 40 min, 82.2% activity remains Pseudoalteromonas sp. ANT506
50
-
purified recombinant His-tagged enzyme, 40 min, 59.4% activity remains Pseudoalteromonas sp. ANT506
70
-
purified recombinant His-tagged enzyme, 20 min, almost no activity remains Pseudoalteromonas sp. ANT506

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant His-tagged enzyme Pseudoalteromonas sp. ANT506

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 over 90% of maximal enzyme activity at pH 7.0-9.0, rapid loss of activity below pH 5.0 and above pH 10.0 Pseudoalteromonas sp. ANT506

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas sp. ANT506 sequence calculation
-
5.09