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Literature summary for 1.15.1.1 extracted from
- Detection, distribution and characterization of novel superoxide dismutases from Yersinia enterocolitica Biovar 1A (2013), PLoS ONE, 8, e63919.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sodA, expression of SodA in and complementation of enzyme-deficient Escherichia coli strain PN134. Expressing YeSodA confers resistance to paraquat to the Escherichia coli strain | Yersinia enterocolitica |
| gene sodB, expression of SodB in and complementation of enzyme-deficient Escherichia coli strain PN134. Expressing YeSodB confers resistance to paraquat to the Escherichia coli strain | Yersinia enterocolitica |
| gene sodC, SodC cannot be expressed in Escherichia coli strain BL21(DE3) | Yersinia enterocolitica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloroform:ethanol solution | the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution; the Mn- and Fe-SODs of Yérsinia enterocolitica are inhibited by chloroform:ethanol solution | Yersinia enterocolitica |  |
| diethyldithiocarbamate | inhibits SodC specifically | Yersinia enterocolitica | |
| additional information | no inhibition by diethyldithiocarbamate; no inhibition by diethyldithiocarbamate | Yersinia enterocolitica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Yersinia enterocolitica | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a Mn-Fe-SOD | Yersinia enterocolitica | |
| Mn2+ | a Mn-Fe-SOD | Yersinia enterocolitica | |
| additional information | binding ligands: His27, His74, Asp157 and His161 in SodB | Yersinia enterocolitica | |
| additional information | binding ligands: His27, His82, Asp169 and His173 in SodA | Yersinia enterocolitica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
SodB, gel filtration | Yersinia enterocolitica |
| 23000 | - |
4 * 23000, recombinant SodA, SDS-PAGE | Yersinia enterocolitica |
| 82000 | - |
SodA, gel filtration | Yersinia enterocolitica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yersinia enterocolitica | A9U908 | gene sodA | - |
| Yersinia enterocolitica | J7H7U2 | gene sodC | - |
| Yersinia enterocolitica | J7HET3 | genes sodA, sodB, and sodC | - |
| Yersinia enterocolitica IP27366 | A9U908 | gene sodA | - |
| Yersinia enterocolitica IP27366 | J7H7U2 | gene sodC | - |
| Yersinia enterocolitica IP27366 | J7HET3 | genes sodA, sodB, and sodC | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12800 | - |
purified recombinant SodA, 4°C, pH 6.0 | Yersinia enterocolitica |
| 24400 | - |
purified recombinant SodB, 4°C, pH 4.0 | Yersinia enterocolitica |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21000, recombinant SodB, SDS-PAGE | Yersinia enterocolitica |
| More | secondary structure analysis using circular dichroism, overview. At pH 7.0 and 28°C, SodA contains 29% alpha-helix and 16% beta-sheets. The elements of secondary structures are more sensitive to pH than to temperature | Yersinia enterocolitica |
| More | secondary structure analysis using circular dichroism, overview. At pH 7.0 and 28°C, SodB contains 44% alpha-helix and 13% beta-sheets. The elements of secondary structures are more sensitive to pH than to temperature | Yersinia enterocolitica |
| tetramer | 4 * 23000, recombinant SodA, SDS-PAGE | Yersinia enterocolitica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SODA | - |
Yersinia enterocolitica |
| SodB | - |
Yersinia enterocolitica |
| SodC | ambiguous | Yersinia enterocolitica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Yersinia enterocolitica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
SodB | Yersinia enterocolitica |
| 6 | - |
SodA | Yersinia enterocolitica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Yersinia enterocolitica | SodB, isoelectric focusing | - |
5.8 |
| Yersinia enterocolitica | SodA, isoelectric focusing | - |
6.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | secondary structure analysis using circular dichroism, overview | Yersinia enterocolitica |
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