Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Meretrix meretrix |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Meretrix meretrix | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu,Zn-superoxide dismutase, residues H46, H48, H63, and H119 are involved in Cu2+ binding | Meretrix meretrix | |
Zn2+ | a Cu,Zn-superoxide dismutase, residues H63, H71, H80, and D83 are involved in Zn2+ binding | Meretrix meretrix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, recombinant His-tagged enzyme, SDS-PAGE | Meretrix meretrix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meretrix meretrix | D2K8K6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Meretrix meretrix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemocyte | low expression rate | Meretrix meretrix | - |
hepatopancreas | high expression rate | Meretrix meretrix | - |
additional information | real-time PCR enzyme expression analysis | Meretrix meretrix | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.12 | - |
purified recombinant His-tagged enzyme, pH 7.4, 25°C | Meretrix meretrix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme assay measures the enzyme's ability to inhibit the oxidation of hydroxylamine catalyzed by the xanthine-xanthine oxidase system | Meretrix meretrix | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, recombinant His-tagged enzyme, SDS-PAGE | Meretrix meretrix |
Synonyms | Comment | Organism |
---|---|---|
Cu/Zn superoxide dismutase | - |
Meretrix meretrix |
Cu/Zn-SOD | - |
Meretrix meretrix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Meretrix meretrix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | purified recombinant enzyme, 10 min, 83% activity within this range remaining | Meretrix meretrix |
90 | - |
purified recombinant enzyme, 10 min, inactivation | Meretrix meretrix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Meretrix meretrix |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | purified recombinant enzyme, 10 min, inactivation | Meretrix meretrix |
2.2 | 10.2 | purified recombinant enzyme, 10 min, 87% activity within this range remaining, rapid loss above pH 10.2 | Meretrix meretrix |
Organism | Comment | Expression |
---|---|---|
Meretrix meretrix | challenge with Vibrio anguillarum increases Mm-icCu/Zn-SOD expression | up |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme sequence contains an intracellular disulfide bond and two Cu/Zn-superoxide dismutase signatures | Meretrix meretrix |
physiological function | the enzyme may play an important role in the innate immune system of hard clam | Meretrix meretrix |