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Literature summary for 1.15.1.1 extracted from

  • Gao, X.; He, C.; Liu, H.; Li, H.; Zhu, D.; Cai, S.; Xia, Y.; Wang, Y.; Yu, Z.
    Intracellular Cu/Zn superoxide dismutase (Cu/Zn-SOD) from hard clam Meretrix meretrix: its cDNA cloning, mRNA expression and enzyme activity (2012), Mol. Biol. Rep., 39, 10713-10722.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Meretrix meretrix

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Meretrix meretrix 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a Cu,Zn-superoxide dismutase, residues H46, H48, H63, and H119 are involved in Cu2+ binding Meretrix meretrix
Zn2+ a Cu,Zn-superoxide dismutase, residues H63, H71, H80, and D83 are involved in Zn2+ binding Meretrix meretrix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
x * 21000, recombinant His-tagged enzyme, SDS-PAGE Meretrix meretrix

Organism

Organism UniProt Comment Textmining
Meretrix meretrix D2K8K6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Meretrix meretrix

Source Tissue

Source Tissue Comment Organism Textmining
hemocyte low expression rate Meretrix meretrix
-
hepatopancreas high expression rate Meretrix meretrix
-
additional information real-time PCR enzyme expression analysis Meretrix meretrix
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.12
-
purified recombinant His-tagged enzyme, pH 7.4, 25°C Meretrix meretrix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme assay measures the enzyme's ability to inhibit the oxidation of hydroxylamine catalyzed by the xanthine-xanthine oxidase system Meretrix meretrix ?
-
?

Subunits

Subunits Comment Organism
? x * 21000, recombinant His-tagged enzyme, SDS-PAGE Meretrix meretrix

Synonyms

Synonyms Comment Organism
Cu/Zn superoxide dismutase
-
Meretrix meretrix
Cu/Zn-SOD
-
Meretrix meretrix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Meretrix meretrix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 purified recombinant enzyme, 10 min, 83% activity within this range remaining Meretrix meretrix
90
-
purified recombinant enzyme, 10 min, inactivation Meretrix meretrix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Meretrix meretrix

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 purified recombinant enzyme, 10 min, inactivation Meretrix meretrix
2.2 10.2 purified recombinant enzyme, 10 min, 87% activity within this range remaining, rapid loss above pH 10.2 Meretrix meretrix

Expression

Organism Comment Expression
Meretrix meretrix challenge with Vibrio anguillarum increases Mm-icCu/Zn-SOD expression up

General Information

General Information Comment Organism
additional information the enzyme sequence contains an intracellular disulfide bond and two Cu/Zn-superoxide dismutase signatures Meretrix meretrix
physiological function the enzyme may play an important role in the innate immune system of hard clam Meretrix meretrix