Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, expression of soluble enzyme in Escherichia coli strain BL21(DE3) | Thermus filiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a cambialistic Mn/Fe-SOD | Thermus filiformis | |
Mn2+ | a cambialistic Mn/Fe-SOD | Thermus filiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Thermus filiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus filiformis | M9QJD2 | - |
- |
Thermus filiformis ATCC 43280 | M9QJD2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Thermus filiformis |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Thermus filiformis |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Thermus filiformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Thermus filiformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
purified recombinant enzyme, inactivation | Thermus filiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.2 | assay at | Thermus filiformis |
General Information | Comment | Organism |
---|---|---|
additional information | the amount of enzyme required to inhibit 50% of pyrogallol autoxidation is 0.41, 0.56 and 13.73 mg at 65°C, 70°C, and 80°C, respectively | Thermus filiformis |