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Literature summary for 1.15.1.1 extracted from
- A highly stable Cu/Zn superoxide dismutase from Withania somnifera plant: gene cloning, expression and characterization of the recombinant protein (2011), Biotechnol. Lett., 33, 2057-2063.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLys | Withania somnifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the purified recombinant enzyme shows a high degree of resistance to detergent, ethanol and protease digestion | Withania somnifera |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Withania somnifera | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a Cu/Zn SOD, 0.83 ng atom Cu per mg protein | Withania somnifera |  |
| additional information | iron, manganese, and nickel contents are below the detection level | Withania somnifera | |
| Zn2+ | a Cu/Zn SOD, 0.41 ng atom Zn per mg protein | Withania somnifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15960 | - |
2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
| 17000 | - |
2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
| 34000 | - |
recombinant enzyme, native PAGE and gel filtration | Withania somnifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Withania somnifera | G0WP59 | RSS-30 chemotype | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme sequence shows the presence of putative sites of disulphide bonds | Withania somnifera |
| no glycoprotein | the enzyme sequence lacks any putative glycosylation sites | Withania somnifera |
| phosphoprotein | the enzyme sequence shows the presence of putative sites of phosphorylation | Withania somnifera |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3)pLys to near homogeneity | Withania somnifera |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4200 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Withania somnifera |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 15960, sequence calculation, 2 * 17000, recombinant enzyme, SDS-PAGE | Withania somnifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cu/Zn SOD | - |
Withania somnifera |
| Cu/Zn superoxide dismutase | - |
Withania somnifera |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
purified recombinant enzyme, 1 h, 90% activity remaining | Withania somnifera |
| 90 | - |
purified recombinant enzyme, 1 h, 70% activity remaining | Withania somnifera |
| 95 | - |
purified recombinant enzyme, 4.68 h, 50% activity remaining | Withania somnifera |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 11 | purified recombinnat enzyme, stable in a broad range | Withania somnifera |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Withania somnifera | sequence calculation | - |
5.33 |
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