Cloned (Comment) | Organism |
---|---|
expression of recombinant chimera MnSOD-VHb in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of a novel chimera of human Mn-SOD and Vitreoscilla hemoglobin, i.e. MnSOD-VHb, for rapid detoxification of reactive oxygen species, the recombinant bifunctional enzyme possesses MnSOD and peroxidase-like activities. The greater antioxidant capability is possibly due to the close proximity between the active site of MnSOD and the heme moiety of VHb, synergistic functions of SOD and peroxidase, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics with artificial substrates, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn | a Mn-SOD | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
4 * 22000, Mn-SOD, SDS-PAGE | Homo sapiens |
38000 | - |
2 * 38000, recombinant chimera MnSOD-VHb, SDS-PAGE | Homo sapiens |
76000 | - |
recombinant chimera MnSOD-VHb, gel filtration | Homo sapiens |
88000 | - |
Mn-SOD, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ + | Homo sapiens | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant chimera MnSOD-VHb from Escherichia coli strain BL21(DE3) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ + | - |
Homo sapiens | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, recombinant chimera MnSOD-VHb, SDS-PAGE | Homo sapiens |
homotetramer | 4 * 22000, Mn-SOD, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
manganese superoxide dismutase | - |
Homo sapiens |
MnSOD | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the chimeric protein MnSOD-VHb is a dimer, in which the two subunits are associated via interaction between the SOD portions, since MnSOD monomers show a 40fold reduction in activity, but our chimeric MnSOD-VHb retained 84% activity compared to native MnSOD | Homo sapiens |