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Literature summary for 1.15.1.1 extracted from
- Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase (2009), Fish Shellfish Immunol., 27, 318-324.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, real-time RT-PCR expression analysis, sequence comparisons, expression in Escherichia coli strain QC779 | Danio rerio |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is stable in presence of 4% SDS | Danio rerio |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn | a MnSOD | Danio rerio |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24989 | - |
x * 24989, sequence calculation, MnSOD | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ | Danio rerio | - |
O2 + H2O2 | - |
? | |
| 2 O2.- + 2 H+ | Danio rerio AB | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q2TV65 | - |
- |
| Danio rerio AB | Q2TV65 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain QC779 | Danio rerio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | MnSOD developmental expression, overview | Danio rerio | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ | - |
Danio rerio | O2 + H2O2 | - |
? | |
| 2 O2.- + 2 H+ | - |
Danio rerio AB | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24989, sequence calculation, MnSOD | Danio rerio |
| More | MnSOD molecular modelling of the highly conserved sequence, structure modelling and comparison with MnSODs from other organisms | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| manganese superoxide dismutase | - |
Danio rerio |
| Mn-SOD | - |
Danio rerio |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal inactivation kinetics fit the first-order inactivation rate equation, recombinant enzyme | Danio rerio |
| 70 | - |
purified recombinant enzyme, half-life is 48 min | Danio rerio |
| 80 | - |
purified recombinant enzyme, 48% remaining activity after 10 min | Danio rerio |
| 90 | - |
purified recombinant enzyme, inactivation after 10 min | Danio rerio |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 11.2 | high enzyme activity | Danio rerio |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Danio rerio | sequence calculation, MnSOD | - |
8.29 |
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