Cloned (Comment) | Organism |
---|---|
gene sodB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Aliivibrio salmonicida |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme by hanging drop vapour diffusion method, 6.5 mg/ml protein in a solution containing 0.1 M Tris-HCl, 1.4 M sodium citrate pH 8.5, 18°C, few days, X-ray diffraction structure determination and analysis at 1.7 A resolution | Aliivibrio salmonicida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | Aliivibrio salmonicida | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio salmonicida | B6ENP9 | gene sodB | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Aliivibrio salmonicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | - |
Aliivibrio salmonicida | O2 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FeSOD | - |
Aliivibrio salmonicida |
iron superoxide dismutase | - |
Aliivibrio salmonicida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
an increased net negative charge on the surface of asFeSOD may explain its lower thermostability compared to the enzyme from Escherichia coli, structure-thermostability relationship, overview | Aliivibrio salmonicida |
57 | - |
purified recombinant enzyme, loss of over 50% activity | Aliivibrio salmonicida |