Literature summary for 1.15.1.1 extracted from

Trinh, C.H.; Hunter, T.; Stewart, E.E.; Phillips, S.E.; Hunter, G.J.
Purification, crystallization and X-ray structures of the two manganese superoxide dismutases from Caenorhabditis elegans (2008), Acta Crystallogr. Sect. F, 64, 1110-1114.

Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged MnSOD-2 and MnSOD-3	Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment)	Organism
MnSOD-2 and MnSOD-3, at 3 and 8 mg/ml respectively, in 10 mM Tris-HCl, pH 7.8, mixing of 0.001 ml protein and reservoir solution, the latter containing 0.1 M bicine pH 9.2 and 3.0 M ammonium sulfate for MnSOD-2, and 0.1 M bicine, pH 9.2, and 2.7 M ammonium sulfate for MnSOD-3, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution	Caenorhabditis elegans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	MnSOD-2 and MnSOD-3	Caenorhabditis elegans	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn	Mn-SOD	Caenorhabditis elegans

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged MnSOD-2 and MnSOD-3 by nickel affinity and anion exchange chromatography, followed by gel filtration	Caenorhabditis elegans

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	MnSOD-2 is constitutively expressed, while synthesis of MnSOD-3 is inducible	Caenorhabditis elegans	-

Synonyms

Synonyms	Comment	Organism
manganese superoxide dismutase	-	Caenorhabditis elegans
MnSOD-2	-	Caenorhabditis elegans
MnSOD-3	-	Caenorhabditis elegans

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Release 2023.1 (January 2023)