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Literature summary for 1.15.1.1 extracted from
- Isolation and characterization of superoxide dismutase from wheat seedlings (2008), J. Agric. Food Chem., 56, 8121-8129.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | at 2.5 mM | Triticum aestivum |  |
| HgCl2 | 1 mM, inhibition of isozyme SODI | Triticum aestivum | |
| additional information | isoyzme SODI is insensitive to H2O2 and KCN | Triticum aestivum | |
| N-ethyl-5-phenylisoxazolium 3'-sulfonate | i.e. Woodwards reagent, Kat 50 mM | Triticum aestivum | |
| SDS | 1 mM, inhibition of isozyme SODI | Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | isozyme SODI is a Mn-SOD | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, isozyme SODI, SDS-PAGE | Triticum aestivum |
| 46000 | - |
isozyme SODI, gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O2.- + H+ | Triticum aestivum | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes SODI and SODII 154fold and 98fold, respectively, from seedlings by heat treatment at 40°C for 15 min, ammonium sulfate fractionation, anionic exchange chromatography, and gel filtration | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 113.8 | - |
purified isozyme SODII | Triticum aestivum |
| 179 | - |
purified isozyme SODI | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme activity determination by NBT reduction | Triticum aestivum | ? | - |
? | |
| O2.- + H+ | - |
Triticum aestivum | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, isozyme SODI, SDS-PAGE | Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Triticum aestivum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 80 | temprature profile, overview | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | purified isozyme SODI, stable | Triticum aestivum |
| 50 | 70 | purified isozyme SODI, inactivation, thermal inactivation of wheat seedling MnSOD follows first-order reaction kinetics, and the temperature dependence of rate constants is in agreement with the Arrhenius equation | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
isozyme SODI | Triticum aestivum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | purified isozyme SODI, stable | Triticum aestivum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Triticum aestivum | isozyme SODI, isoelectric focusing | - |
4 |
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