Cloned (Comment) | Organism |
---|---|
gene sodC, subcloning in Escherichia coli, complementation of the sodC mutant, expression of a SodC-FLAG fusion protein in strain K56-2 | Burkholderia cenocepacia |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme null mutant cell is not sensitive to H2O2, 3-morpholinosydnonimine, or paraquat challenge, but is killed by exogenous superoxide generated by the xanthine/xanthine oxidase method | Burkholderia cenocepacia |
additional information | construction of a sodC null mutant strain KEK1, which is not sensitive to a H2O2, 3-morpholinosydnonimine, or paraquat challenge, but is killed by exogenous superoxide generated by the xanthine/xanthine oxidase method. The sodC mutant also exhibits a growth defect in liquid medium compared to the parental strain, which can be complemented in trans. The mutant organism is killed more rapidly than the parental strain in murine macrophage-like cell line RAW 264.7, but killing is eliminated when macrophages are treated with an NADPH oxidase inhibitor, overview | Burkholderia cenocepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not inhibited by H2O2 and unusually resistant to KCN; SodC is resistant to inhibition by H2O2 and is unusually resistant to KCN for a Cu,Zn-SOD | Burkholderia cenocepacia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Burkholderia cenocepacia | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Burkholderia cenocepacia | |
Cu2+ | a Cu,ZnSOD | Burkholderia cenocepacia | |
Zinc | - |
Burkholderia cenocepacia | |
Zn2+ | a Cu,ZnSOD | Burkholderia cenocepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Burkholderia cenocepacia | periplasmic Cu,ZnSOD protects the bacterium from exogenously generated O2.- and contributes to intracellular survival of the bacterium in macrophages | O2 + H2O2 | - |
? | |
O2.- + H+ | Burkholderia cenocepacia K56-2 | periplasmic Cu,ZnSOD protects the bacterium from exogenously generated O2.- and contributes to intracellular survival of the bacterium in macrophages | O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cenocepacia | - |
- |
- |
Burkholderia cenocepacia K56-2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | - |
Burkholderia cenocepacia | O2 + H2O2 | - |
? | |
O2.- + H+ | periplasmic Cu,ZnSOD protects the bacterium from exogenously generated O2.- and contributes to intracellular survival of the bacterium in macrophages | Burkholderia cenocepacia | O2 + H2O2 | - |
? | |
O2.- + H+ | - |
Burkholderia cenocepacia K56-2 | O2 + H2O2 | - |
? | |
O2.- + H+ | periplasmic Cu,ZnSOD protects the bacterium from exogenously generated O2.- and contributes to intracellular survival of the bacterium in macrophages | Burkholderia cenocepacia K56-2 | O2 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cu,ZnSOD | - |
Burkholderia cenocepacia |
SodC | - |
Burkholderia cenocepacia |