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Literature summary for 1.15.1.1 extracted from

  • Carter, C.; Thornburg, R.W.
    Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues (2000), J. Biol. Chem., 275, 36726-36733.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CN- no inhibition Mn-SOD Nicotiana sp.
H2O2 no inhibition: Mn-SOD Nicotiana sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular Mn-SOD, nectar Nicotiana sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ specific for Nicotiana sp.
Mn2+ 0.5 mol per mol of subunit Nicotiana sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
165000
-
nectarin I: Mn-SOD, native PAGE Nicotiana sp.

Organism

Organism UniProt Comment Textmining
Nicotiana sp.
-
nectarin I with Mn-SOD activity
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein biantennary nonasaccharide with composition (GlnNAc)4:Man3:Xyl:Fuc Nicotiana sp.
glycoprotein nectarin I: Mn-SOD Nicotiana sp.

Purification (Commentary)

Purification (Comment) Organism
nectarin I: Mn-SOD from nectar Nicotiana sp.

Renatured (Commentary)

Renatured (Comment) Organism
activity cannot be restored by Fe2+, Cu2+, Zn2+, and Cu2+/Zn2+, but by Mn2+ Nicotiana sp.

Source Tissue

Source Tissue Comment Organism Textmining
nectar nectarin I Nicotiana sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Nicotiana sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
Nicotiana sp. O2 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 22500-29000, nectarin I: Mn-SOD, SDS-PAGE and mass spectroscopy Nicotiana sp.
More enzyme is only active as tetramer or pentamer Nicotiana sp.

Synonyms

Synonyms Comment Organism
nectarin I
-
Nicotiana sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
nectarin I: Mn-SOD, 85% remaining activity after 1 h Nicotiana sp.
100
-
complete loss of activity Nicotiana sp.