Cloned (Comment) | Organism |
---|---|
Cu,Zn-SOD, overexpression in Escherichia coli | Anas platyrhynchos domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Anas platyrhynchos domestica | |
Zn2+ | - |
Anas platyrhynchos domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
2 * 16000, Cu,Zn-SOD, SDS-PAGE | Anas platyrhynchos domestica |
32000 | - |
gel filtration | Anas platyrhynchos domestica |
32000 | - |
Cu,Zn-SOD | Anas platyrhynchos domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anas platyrhynchos domestica | - |
Cu,Zn-SOD | - |
Anas platyrhynchos domestica | - |
Peking duck | - |
Anas platyrhynchos domestica CuZn-SOD | - |
Cu,Zn-SOD | - |
Purification (Comment) | Organism |
---|---|
Cu,Zn-SOD, recombinant from Escherichia coli and native enzyme | Anas platyrhynchos domestica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 superoxide + 2 H+ = O2 + H2O2 | amino acid sequence alignment and comparison | Anas platyrhynchos domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Anas platyrhynchos domestica | O2 + H2O2 | - |
? | |
O2- + H+ | - |
Anas platyrhynchos domestica CuZn-SOD | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 16000, Cu,Zn-SOD, SDS-PAGE | Anas platyrhynchos domestica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant Cu,Zn-SOD, after 2 h 20% activity remaining, after 3 h all activity is lost | Anas platyrhynchos domestica |