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Literature summary for 1.15.1.1 extracted from
- Purification and characterization of a manganese-containing superoxide dismutase from a carboxydobacterium, Pseudomonas carboxydohydrogena (1997), Mol. Cells, 7, 730-737.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | no inhibition | Pseudomonas carboxydohydrogena |  |
| H2O2 | no inhibition: Mn-SOD | Pseudomonas carboxydohydrogena | |
| N3- | Mn-SOD | Pseudomonas carboxydohydrogena | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1.1 mol per mol of enzyme | Pseudomonas carboxydohydrogena | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21700 | - |
2 * 21700, Mn-SOD, SDS-PAGE | Pseudomonas carboxydohydrogena |
| 42500 | - |
Mn-SOD, gel filtration | Pseudomonas carboxydohydrogena |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas carboxydohydrogena | - |
Mn-SOD | - |
| Pseudomonas carboxydohydrogena | - |
strain DSM 1083 | - |
| Pseudomonas carboxydohydrogena Mn-SOD | - |
Mn-SOD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mn-SOD | Pseudomonas carboxydohydrogena |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 superoxide + 2 H+ = O2 + H2O2 | amino acid composition, comparison | Pseudomonas carboxydohydrogena |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas carboxydohydrogena |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O2- + H+ | - |
Pseudomonas carboxydohydrogena | O2 + H2O2 | - |
? | |
| O2- + H+ | - |
Pseudomonas carboxydohydrogena Mn-SOD | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 21700, Mn-SOD, SDS-PAGE | Pseudomonas carboxydohydrogena |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas carboxydohydrogena |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
88.5% remaining activity after 1 h | Pseudomonas carboxydohydrogena |
| 70 | - |
complete loss of activity after 40 min | Pseudomonas carboxydohydrogena |
| 90 | - |
complete loss of activity after 5 min | Pseudomonas carboxydohydrogena |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
Mn-SOD | Pseudomonas carboxydohydrogena |
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