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Literature summary for 1.15.1.1 extracted from
- Human liver manganese superoxide dismutase. Purification and crystallization, subunit association and sulfhydryl reactivity (1990), Eur. J. Biochem., 194, 713-720.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme 10 mg per ml in Tris/HCl 50 mM, pH 8.2 by dialysis against ammonium sulfate 2.8 M, pH 8.2, 4°C | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| SDS, 1%, complete loss of activity after 6 h | Homo sapiens |
| urea: 8 M, stable | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | Mn-SOD | Homo sapiens |  |
| iodoacetamide | Mn-SOD | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
4 * 21300, SDS-PAGE | Homo sapiens |
| 88000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
Mn-SOD | - |
| Homo sapiens Mn-SOD | - |
Mn-SOD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mn-SOD from liver | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -35°C, protein concentration 45 mg/ml, 50% glycerol | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O2- + H+ | - |
Homo sapiens | O2 + H2O2 | - |
? | |
| O2- + H+ | - |
Homo sapiens Mn-SOD | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 21300, SDS-PAGE | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
5°C, 1 day, 35% loss of activity | Homo sapiens |
| 7.2 | - |
4°C, 1 day, 2% loss of activity | Homo sapiens |
| 8 | 9.3 | 4°C, 1 day, 10-20% loss of activity | Homo sapiens |
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