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Literature summary for 1.14.99.B10 extracted from

  • Kojima, Y.; Várnai, A.; Ishida, T.; Sunagawa, N.; Petrovic, D.M.; Igarashi, K., Jellison, J.; Goodell, B.; Alfredsen, G.; Westereng, B.; Eijsink, V.G.; Yoshida, M.
    A lytic polysaccharide monooxygenase with broad xyloglucan specificity from the brown-rot fungus Gloeophyllum trabeum and its action on cellulose-xyloglucan complexes (2016), Appl. Environ. Microbiol., 82, 6557-6572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.B10

Cloned(Commentary)

Cloned (Comment) Organism
expression of splice variant LPMO9A-2 in Pichia pastoris Gloeophyllum trabeum
expression of splice variant LpmoA-2 in Pichia pastoris Gloeophyllum trabeum

Inhibitors

Inhibitors Comment Organism Structure
additional information hemicellulose coating has an inhibitory effect on cellulolytic LPMO activity Gloeophyllum trabeum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25100
-
-
 Gloeophyllum trabeum
25800
-
-
 Gloeophyllum trabeum
33100
-
 x * 33100, calculated Gloeophyllum trabeum
36000
-
-
 Gloeophyllum trabeum
60000
-
-
 Gloeophyllum trabeum
60000
-
 gel filtration Gloeophyllum trabeum

Organism

Organism UniProt Comment Textmining
Gloeophyllum trabeum
-
-
-
Gloeophyllum trabeum A0A1C9ZMC3 isoform LPMO9C
-
Gloeophyllum trabeum A0A1C9ZMC5 splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function
-
Gloeophyllum trabeum A0A1C9ZP88 splice variant LPMO9A-1
-
Gloeophyllum trabeum A0A1C9ZUN9 isoform LPMO9D
-
Gloeophyllum trabeum NBRC 6430 A0A1C9ZMC3 isoform LPMO9C
-
Gloeophyllum trabeum NBRC 6430 A0A1C9ZMC5 splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function
-
Gloeophyllum trabeum NBRC 6430 A0A1C9ZP88 splice variant LPMO9A-1
-
Gloeophyllum trabeum NBRC 6430 A0A1C9ZUN9 isoform LPMO9D
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein treatment with EndoH leads to a decrease in molecular mass from 60 kDA to 57 kDa Gloeophyllum trabeum
glycoprotein treatment with EndoH leads to a decrease in molecular mass from 60000 Da to 57000 Da Gloeophyllum trabeum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + dithiothreitol + O2
-
 Gloeophyllum trabeum carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
carboxymethyl cellulose + dithiothreitol + O2 reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan Gloeophyllum trabeum carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
carboxymethyl cellulose + dithiothreitol + O2 reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan Gloeophyllum trabeum NBRC 6430 carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
cellulose + dithiothreitol + O2
-
 Gloeophyllum trabeum oligosaccharides + dithiothreitol disulfide + H2O
-
 ?
glucomannan + ascorbate + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dehydroascorbate + H2O
-
 ?
glucomannan + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 cellooligosaccharides + dehydroascorbate + H2O
-
 ?
glucomannan + dithiothreitol + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dithiothreitol disulfide + H2O minor activity on glucomannan ?
additional information inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required Gloeophyllum trabeum ?
-
 ?
additional information isoform LpmoA-2 has broad specificity, cleaving at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Inactive on shorter cello-oligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan Gloeophyllum trabeum ?
-
 ?
additional information inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required Gloeophyllum trabeum NBRC 6430 ?
-
 ?
phosphoric acid swollen cellulose + ascorbate + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dehydroascorbate + H2O
-
 ?
phosphoric acid swollen cellulose + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 cellooligosaccharides + dehydroascorbate + H2O
-
 ?
xyloglucan + ascorbate + O2
-
 Gloeophyllum trabeum ? + dehydroascorbate + H2O releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides ?
xyloglucan + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 ? + dehydroascorbate + H2O releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides ?
xyloglucan + dithiothreitol + O2 enzyme acts preferentially on free xyloglucan with release of a broad range of xyloglucan oligosaccharides Gloeophyllum trabeum xyloglucan oligosaccharides + dithiothreitol disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 33100, calculated Gloeophyllum trabeum
? x * 25100, calculated Gloeophyllum trabeum
? x * 25800, calculated Gloeophyllum trabeum
? x * 36000, splicing variant Lpmo9A-2, calculated Gloeophyllum trabeum
? x * 60000, SDS-PAGE, x * 36000, calculated Gloeophyllum trabeum

Synonyms

Synonyms Comment Organism
LPMO9D
-
 Gloeophyllum trabeum

pI Value

Organism Comment pI Value Maximum pI Value
Gloeophyllum trabeum calculated
-
 4.6
Gloeophyllum trabeum splicing variant Lpmo9A-2, calculated
-
 4.6
Gloeophyllum trabeum calculated
-
 4.7
Gloeophyllum trabeum calculated
-
 4.9
Gloeophyllum trabeum calculated
-
 5.8