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Literature summary for 1.14.99.69 extracted from

  • Subedi, B.; Corder, A.; Zhang, S.; Foss, F.; Pierce, B.
    Steady-state kinetics and spectroscopic characterization of enzyme-tRNA interactions for the non-heme diiron tRNA-monooxygenase, MiaE (2015), Biochemistry, 54, 363-376 .
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
 i6A-ACSLTrp pH 7.5, 22°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Iron nonheme diiron enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q08015
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
i6A + reduced acceptor + O2 i.e. free nucleoside Salmonella enterica subsp. enterica serovar Typhimurium io6A + acceptor + H2O
-
 ?
i6A-ACSLTrp + reduced acceptor + O2 i.e. isopentenylated anticodon stem loop (ACSL) region of tRNATrp Salmonella enterica subsp. enterica serovar Typhimurium io6A-ACSL-Trp + acceptor + H2O
-
 ?
additional information steady-state product formation retains more than 98%stoichiometric E stereoselectivity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-
N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine37 in tRNA + reduced acceptor + O2
-
 Salmonella enterica subsp. enterica serovar Typhimurium N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 i6A-ACSLTrp pH 7.5, 22°C Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information Comment Organism
physiological function binding of tRNA to MiaE induces a protein conformational change that influences the electronic structure of the diiron site analogous to what has been observed for various bacterial multicomponent diiron monooxygenases Salmonella enterica subsp. enterica serovar Typhimurium