Literature summary for 1.14.99.69 extracted from

Corder, A.; Subedi, B.; Zhang, S.; Dark, A.; Foss Jr., F.; Pierce, B.
Peroxide-shunt substrate-specificity for the Salmonella typhimurium O 2-dependent tRNA modifying monooxygenase (MiaE) (2013), Biochemistry, 52, 6182-6196 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, as N-terminal fusion with maltose-binding protein	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	2.1 iron atoms per MiaE protein	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	gel filtration and analytical ultracentrifugation	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	Q08015	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-chloro-N6-isopentenyladenosine + reduced acceptor + O2	-	Salmonella enterica subsp. enterica serovar Typhimurium	2-chloro-N6-((E)-4-hydroxyisopentenyl)-adenosine + acceptor + H2O	-	?
additional information	enzyme additionally shows catalase activity. Formation of 1.1 mol of O2 is observed upon addition of 2 mol H2O2	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
additional information	hydroxylation of the terminal isopentenyl-C4-position is observed with more than 97% E-stereoselectivity. No other nonspecific hydroxylation products are observed in enzymatic assays. The initial rate of MiaE hydroxylation is highly influenced by the substituent at the C2-position of the nucleoside base. MiaE exhibits more than 3fold enhancement for the hydroxylation of the free ms2i6A nucleoside relative to i6A	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
N6-isopentenyladenosine + reduced acceptor + O2	-	Salmonella enterica subsp. enterica serovar Typhimurium	N6-((E)-4-hydroxyisopentenyl)-adenosine + acceptor + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 25000, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)