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Literature summary for 1.14.99.61 extracted from

  • Görner, C.; Schrepfer, P.; Redai, V.; Wallrapp, F.; Loll, B.; Eisenreich, W.; Haslbeck, M.; Brück, T.
    Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids (2016), Microb. Cell Fact., 15, 86 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.61

Application

Application Comment Organism
synthesis CotB3 can be employed to functionalize other diterpene olefins, such as (-)-casbene to yield sinularcasbane D Streptomyces melanosporofaciens
synthesis efficient heterologous reconstitution of the cyclooctatin biosynthesis cluster by introduction of a novel, non-native Streptomyces-derived redox system (reductase/ferredoxin system from Streptomyces afghaniensis). The complete, heterologous biosynthesis cascade is established in Escherichia coli. The microbial production system allows for a 43fold increased cyclooctatin yield compared to the native producer Streptomyces melanosporofaciens Streptomyces melanosporofaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Streptomyces melanosporofaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclooctat-9-en-7-ol + reduced acceptor + O2 Streptomyces melanosporofaciens the enzyme is involved in the biosynthesis of cyclooctatin cyclooctat-9-ene-5,7-diol + acceptor + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Streptomyces melanosporofaciens C9K1X6
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclooctat-9-en-7-ol + reduced acceptor + O2 the enzyme is involved in the biosynthesis of cyclooctatin Streptomyces melanosporofaciens cyclooctat-9-ene-5,7-diol + acceptor + H2O
-
 ?
cyclooctat-9-en-7-ol + reduced acceptor + O2 redox partner promiscuity of CotB3. The reductase/ferredoxin system from Streptomyces afghaniensis (AfR/Afx) is superior in activating the cylcooctatin-specific class I P450s CotB3/CotB4 compared to the conventional Pseudomonas putida reductase/ferredoxin system (PdR/Pdx) Streptomyces melanosporofaciens cyclooctat-9-ene-5,7-diol + acceptor + H2O
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 ?

Synonyms

Synonyms Comment Organism
CotB3
-
 Streptomyces melanosporofaciens

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Streptomyces melanosporofaciens

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of cyclooctatin Streptomyces melanosporofaciens