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Literature summary for 1.14.99.54 extracted from
- Monitoring of reactions catalyzed by lytic polysaccharide monooxygenases using highly-sensitive fluorimetric assay of the oxygen consumption rate (2017), Carbohydr. Res., 452, 156-161 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothelomyces thermophilus |
| analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Thermothielavioides terrestris |
| analysis | feeding the dissolved reagents into the reaction system during measurements with obtaining a simultaneous response in the oxygen consumption rate allows in situ monitoring the LPMO inhibition and activation by EDTA and Cu2+ ions as well as studying other effects on the enzymatic reaction | Trichoderma reesei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 2 mM, complete loss of activity | Thermothielavioides terrestris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | EDTA-inhibited enzyme can be reactivated by 20 mM Cu2+ within 1 h | Thermothielavioides terrestris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | A0A0H4K9X4 | - |
- |
| Thermothielavioides terrestris | D0VWZ9 | - |
- |
| Trichoderma reesei | O14405 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + ascorbic acid + O2 | - |
Thermothelomyces thermophilus | ? + dehydroascorbate + H2O | - |
? | |
| cellulose + ascorbic acid + O2 | - |
Thermothielavioides terrestris | ? + dehydroascorbate + H2O | - |
? | |
| cellulose + ascorbic acid + O2 | - |
Trichoderma reesei | ? + dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel61a | - |
Trichoderma reesei |
| gh61e | - |
Thermothielavioides terrestris |
| LPMO9A | - |
Thermothelomyces thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
cellulose | pH 5.0, 37°C | Thermothielavioides terrestris | |
| 0.021 | - |
cellulose | pH 5.0, 37°C | Thermothelomyces thermophilus | |
| 0.021 | - |
cellulose | pH 5.0, 37°C | Trichoderma reesei | |
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Release 2023.1 (January 2023)
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