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Literature summary for 1.14.99.54 extracted from

  • Frommhagen, M.; Koetsier, M.J.; Westphal, A.H.; Visser, J.; Hinz, S.W.; Vincken, J.P.; van Berkel, W.J.; Kabel, M.A.; Gruppen, H.
    Lytic polysaccharide monooxygenases from Myceliophthora thermophila C1 differ in substrate preference and reducing agent specificity (2016), Biotechnol. Biofuels, 9, 186.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.54

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermothelomyces thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
comparison of isoforms LPMO9A, LPMO9B and LPMO9C. LPMO9B contains distal from the coordinated copper sphere an additional loop (Gly115-Asn121), which is not present in LPMO9A and LPMO9C. The copper ion in LPMO9A, LPMO9B and LPMO9C is coordinated by His1-His68-Tyr153, His1-His79-Tyr170 and His1-His84-Tyr166, respectively. All three LPMOs share two putative disulfide bridges Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Thermothelomyces thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
-
 Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus A0A0H4K9X4
-
-
Thermothelomyces thermophilus A0A1C9CXI1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-(1->3,1->4)-glucan + acceptor + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized glucan oligosaccharides + reduced acceptor + H2O
-
 ?
cellulose + ascorbate + O2 substrate is regenerated amorphous cellulose Thermothelomyces thermophilus C1-oxidized cellooligosaccharides + cellooligosaccharides + dehydroascorbate + H2O release of C1-oxidized and non-oxidized glucooligosaccharides ?
cellulose + ascorbate + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?
cellulose + oxidized dopamine + O2
-
 Thermothelomyces thermophilus C1-oxidized gluco-oligosaccharides + glucooligosaccharides + dopamine + H2O dopamine shows 46% of the activity with ascorbate ?
additional information for isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid Thermothelomyces thermophilus ?
-
 ?
additional information no substrate: beta-(1->3, 1->4)-glucan. For isoforms LPMO9A, LPMO9B and LPMO9C, ascorbic acid is one of the best electron donors. Besides ascorbic acid, compounds bearing a 1,2-benzenediol moiety such as 3-methylcatechol, 3,4-dihydroxyphenylalanine, or a 1,2,3-benzenetriol moiety such as gallic acid, epigallocatechin-gallate give the highest formation of oxidized and non-oxidized gluco-oligosaccharides. Sinapic acid actes as donor. No electron donor: quercetin or taxifolin, and tannic acid Thermothelomyces thermophilus ?
-
 ?
xylan + dopamine + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized xylo-oligosaccharides + 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O 93% of the activiy with ascorbate ?
xyloglucan + acceptor + O2
-
 Thermothelomyces thermophilus C1/C4-oxidized oxidized oligosaccharides + reduced acceptor + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 30633, calculated Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
LPMO9A
-
 Thermothelomyces thermophilus
LPMO9B
-
 Thermothelomyces thermophilus