Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mol of copper per mol of proein | Neurospora crassa | |
Cu2+ | 1 mol of copper per mol of protein | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q1K8B6 | isoform endoglucanase II | - |
Neurospora crassa | Q7RWN7 | isoform endoglucanase II | - |
Neurospora crassa | Q7SA19 | isoform endoglucanase IV | - |
Neurospora crassa ATCC 24698 | Q1K8B6 | isoform endoglucanase II | - |
Neurospora crassa ATCC 24698 | Q7RWN7 | isoform endoglucanase II | - |
Neurospora crassa ATCC 24698 | Q7SA19 | isoform endoglucanase IV | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 1-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose | Neurospora crassa | ? | - |
? | |
additional information | mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 4-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose | Neurospora crassa | ? | - |
? | |
additional information | mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 4-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose | Neurospora crassa ATCC 24698 | ? | - |
? | |
additional information | mechanism may follow one electron reduction of PMO-Cu(II) to PMO-Cu(I) by the cellobiose dehydrogenase heme domain followed by oxygen binding and internal electron transfer to form a copper superoxo intermediate. Hydrogen atom abstraction by the copper superoxo at the 1-position of an internal carbohydrate then takes place, generating a copper hydroperoxo intermediate and a substrate radical. The second electron from cellobiose dehydrogenase then facilitates O-O bond cleavage releasing water and generating a copper oxo radical that couples with the substrate radical, thereby hydroxylating the polysaccharide. The additional oxygen atom destabilizes the glycosidic bond leading to elimination of the adjacent glucan and formation of a sugar lactone or ketoaldose | Neurospora crassa ATCC 24698 | ? | - |
? | |
phosphoric acid swollen cellulose + AH2 + O2 | - |
Neurospora crassa | ? + dehydroascorbate + H2O | in presence of cellobiose dehydrogenase, products include doubly oxidized cellodextrin | ? | |
phosphoric acid swollen cellulose + AH2 + O2 | - |
Neurospora crassa ATCC 24698 | ? + dehydroascorbate + H2O | in presence of cellobiose dehydrogenase, products include doubly oxidized cellodextrin | ? |
Synonyms | Comment | Organism |
---|---|---|
endoglucanase II | - |
Neurospora crassa |
endoglucanase IV | - |
Neurospora crassa |
gh61-4 | - |
Neurospora crassa |
gh61-5 | - |
Neurospora crassa |
NCU07898 | - |
Neurospora crassa |
PMO | - |
Neurospora crassa |
polysaccharide monoxygenase | - |
Neurospora crassa |
General Information | Comment | Organism |
---|---|---|
physiological function | cellobiose dehydrogenase enhances cellulose degradation by coupling the oxidation of cellobiose to the reductive activation of copper-dependent polysaccharide monooxygenase that catalyzes the insertion of oxygen into C-H bonds adjacent to the glycosidic linkage | Neurospora crassa |