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Literature summary for 1.14.99.53 extracted from

  • Forsberg, Z.; Nelson, C.E.; Dalhus, B.; Mekasha, S.; Loose, J.S.; Crouch, L.I.; Rohr, A.K.; Gardner, J.G.; Eijsink, V.G.; Vaaje-Kolstad, G.
    Structural and functional analysis of a lytic polysaccharide monooxygenase important for efficient utilization of chitin in Cellvibrio japonicus (2016), J. Biol. Chem., 291, 7300-7312.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.53

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cellvibrio japonicus

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.85 A resolution, tri-modular enzyme containing a catalytic family AA10 LPMO module, a family 5 chitin-binding module, and a C-terminal unclassified module which displays tight and specific binding to chitin Cellvibrio japonicus

Protein Variants

Protein Variants Comment Organism
additional information removal of the chitin-binding modules reduces LPMO activity toward alpha-chitin. The full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well Cellvibrio japonicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the active site contains a copper ion coordinated by residues His-37 and His-136 in a T-shaped histidine brace Cellvibrio japonicus

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus B3PJ79
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + ascorbate + O2
-
 Cellvibrio japonicus chito-oligosaccharide aldonic acid + dehydroascorbate + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ?
beta-chitin + acceptor + O2
-
 Cellvibrio japonicus chito-oligosaccharide aldonic acid + reduced acceptor + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ?

Synonyms

Synonyms Comment Organism
Cbp33A
-
 Cellvibrio japonicus
LPMO10A
-
 Cellvibrio japonicus

General Information

General Information Comment Organism
physiological function the full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well, also yielding similar amounts of oxidized products. When grown on insoluble chitin substrates, the deletion mutant shows a reproducible growth defect on beta-chitin leading to an 2fold slower growth rate compared with the wild type strain. When grown on crab shells, the mutation leads to an extended lag phase of about 100 h Cellvibrio japonicus