Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | an iron-dependent sulfoxide synthase | Microcystis aeruginosa | |
Fe2+ | an iron-dependent sulfoxide synthase | Moorena producens | |
Fe2+ | an iron-dependent sulfoxide synthase | Erwinia tasmaniensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hercynine + gamma-L-glutamyl-L-cysteine + O2 | Moorena producens | - |
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O | - |
? | |
hercynine + gamma-L-glutamyl-L-cysteine + O2 | Moorena producens PAL-8-15-08-1 | - |
gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | Moorena producens | - |
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | Moorena producens PAL-8-15-08-1 | - |
S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
additional information | Moorena producens | an OvoA-like protein, full-length OvoA homologue, OvoA_2, is a monofunctional sulfoxide synthase | ? | - |
? | |
additional information | Microcystis aeruginosa | short cyanobacterial OvoA-type enzymes may contribute to ergothioneine (EC 1.14.99.51) instead of ovothiol production | ? | - |
? | |
additional information | Erwinia tasmaniensis | the ovothiol biosynthetic sulfoxide synthase OvoA from Erwinia tasmaniensis (OvoAErwin) is a promiscuous enzyme. This enzyme is most efficient in making its native product S-(L-histidin-5-yl)-L-cysteine S-oxide, but, when presented with N-alpha-trimethylhistidine as a sulfur acceptor, the enzyme switches product specificity and produces gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide albeit with significantly lower efficiency | ? | - |
? | |
additional information | Moorena producens PAL-8-15-08-1 | an OvoA-like protein, full-length OvoA homologue, OvoA_2, is a monofunctional sulfoxide synthase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Erwinia tasmaniensis | - |
- |
- |
Microcystis aeruginosa | - |
- |
- |
Moorena producens | A0A1D8U0C7 | - |
- |
Moorena producens | A0A1D8U386 | - |
- |
Moorena producens PAL-8-15-08-1 | A0A1D8U0C7 | - |
- |
Moorena producens PAL-8-15-08-1 | A0A1D8U386 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hercynine + gamma-L-glutamyl-L-cysteine + O2 | - |
Moorena producens | gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O | - |
? | |
hercynine + gamma-L-glutamyl-L-cysteine + O2 | - |
Moorena producens PAL-8-15-08-1 | gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | - |
Microcystis aeruginosa | S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | - |
Moorena producens | S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | - |
Erwinia tasmaniensis | S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
L-histidine + L-cysteine + O2 | - |
Moorena producens PAL-8-15-08-1 | S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O | - |
? | |
additional information | an OvoA-like protein, full-length OvoA homologue, OvoA_2, is a monofunctional sulfoxide synthase | Moorena producens | ? | - |
? | |
additional information | short cyanobacterial OvoA-type enzymes may contribute to ergothioneine (EC 1.14.99.51) instead of ovothiol production | Microcystis aeruginosa | ? | - |
? | |
additional information | the ovothiol biosynthetic sulfoxide synthase OvoA from Erwinia tasmaniensis (OvoAErwin) is a promiscuous enzyme. This enzyme is most efficient in making its native product S-(L-histidin-5-yl)-L-cysteine S-oxide, but, when presented with N-alpha-trimethylhistidine as a sulfur acceptor, the enzyme switches product specificity and produces gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide albeit with significantly lower efficiency | Erwinia tasmaniensis | ? | - |
? | |
additional information | in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErwin produces only S-(L-histidin-5-yl)-L-cysteine S-oxide, whereas OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide | Moorena producens | ? | - |
? | |
additional information | an OvoA-like protein, full-length OvoA homologue, OvoA_2, is a monofunctional sulfoxide synthase | Moorena producens PAL-8-15-08-1 | ? | - |
? | |
additional information | in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErwin produces only S-(L-histidin-5-yl)-L-cysteine S-oxide, whereas OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide | Moorena producens PAL-8-15-08-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OvoA | - |
Moorena producens |
OvoA-like protein | - |
Microcystis aeruginosa |
OvoA-like protein | - |
Moorena producens |
OvoAErwin | - |
Erwinia tasmaniensis |
OvoA_1 | - |
Moorena producens |
OvoA_2 | - |
Moorena producens |
short OvoA homologue | - |
Microcystis aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | cyanobacterial OvoA homologues (Egt-B(ovo)) have evolved to catalyze an EgtB-type reaction by convergent evolution, cf. EC 1.14.99.50, in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide | Microcystis aeruginosa |
evolution | cyanobacterial OvoA homologues (Egt-B(ovo)) have evolved to catalyze an EgtB-type reaction by convergent evolution, cf. EC 1.14.99.50, in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide | Moorena producens |
evolution | cyanobacterial OvoA homologues (Egt-B(ovo)) have evolved to catalyze an EgtB-type reaction by convergent evolution, cf. EC 1.14.99.50, in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide. Because Erwinia tasmaniensis and most other OvoA encoding organisms do not encode an EgtD-type histidine methyltransferase, it seems clear that this N-alpha-trimethylhistidine-consuming side activity of OvoAErwin has no physiological purpose. Such promiscuity may have facilitated the transition of an ancestral sulfoxide synthase from ovothiol to erothioneine biosynthesis | Erwinia tasmaniensis |
metabolism | sulfoxide synthase OvoA catalyzes the formation of 5-histidylcysteine sulfoxide in the ovothiol biosynthesis. Short cyanobacterial OvoA-type enzymes may contribute to ergothioneine (EC 1.14.99.51) instead of ovothiol production | Microcystis aeruginosa |
metabolism | sulfoxide synthase OvoA catalyzes the formation of 5-histidylcysteine sulfoxide in the ovothiol biosynthesis. Short cyanobacterial OvoA-type enzymes may contribute to ergothioneine (EC 1.14.99.51) instead of ovothiol production | Moorena producens |
metabolism | sulfoxide synthase OvoA catalyzes the formation of 5-histidylcysteine sulfoxide in the ovothiol biosynthesis. Short cyanobacterial OvoA-type enzymes may contribute to ergothioneine (EC 1.14.99.51) instead of ovothiol production | Erwinia tasmaniensis |
additional information | an OvoA-like protein, full-length OvoA homologue, OvoA_1, with a C-terminal methyltransferase, most OvoAs contain a C-terminal methyltransferase | Moorena producens |
additional information | homologue OvoA_2 is a monofunctional sulfoxide synthase without a C-terminal methyltransferase | Moorena producens |