Cloned (Comment) | Organism |
---|---|
expresssion in Escherichia coli, cultures overexpressing isoforms IsdG or IsdI exhibit a bright yellow color | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Staphylococcus aureus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12500 | - |
x * 12500, recombinant His-tagged protein, SDS-PAGE | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoheme + 4 AH2 + 4 O2 | Staphylococcus aureus | - |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | Staphylococcus aureus | - |
5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | Staphylococcus aureus N315 | - |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | Staphylococcus aureus N315 | - |
5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q7A649 | isoform IsdG | - |
Staphylococcus aureus | Q7A827 | isoform IsdI | - |
Staphylococcus aureus N315 | Q7A649 | isoform IsdG | - |
Staphylococcus aureus N315 | Q7A827 | isoform IsdI | - |
Renatured (Comment) | Organism |
---|---|
reconstitution of both isoform IsdG and IsdI with heme at pH 8.0 generates optical absorption spectra containing a Soret band at about 412 nm, and alpha/beta bands at about 567 and 532 nm | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoheme + 4 AH2 + 4 O2 | - |
Staphylococcus aureus | 15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | - |
Staphylococcus aureus N315 | 15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | - |
Staphylococcus aureus | 5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? | |
protoheme + 4 AH2 + 4 O2 | - |
Staphylococcus aureus N315 | 5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O | enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 12500, recombinant His-tagged protein, SDS-PAGE | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform IsdI complements the heme utilization deficiency of a Corynebacterium ulcerans heme oxygenase mutant | Staphylococcus aureus |