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Literature summary for 1.14.99.39 extracted from

  • Miyaji, A.; Miyoshi, T.; Motokura, K.; Baba, T.
    Discrimination of the prochiral hydrogens at the C-2 position of n-alkanes by the methane/ammonia monooxygenase family proteins (2015), Org. Biomol. Chem., 13, 8261-8270 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nitrosomonas europaea enzyme AMO-Ne in whole cells produces 1- and 2-alcohols from C4-C8n-alkanes, the regioselectivity is dependent on the length of the carbon chain. 2-Alcohols produced from C4-C7n-alkanes are predominantly either the R- or S-enantiomers, while 2-octanol produced from n-octane is racemic. AMO-Ne can discriminate between the prochiral hydrogens at the C-2 position, with the degree of discrimination varying according to the n-alkane, and AMO-Ne shows a distinct ability to discriminate between the orientation of n-butane and n-pentane in the catalytic site, as compared to the particulate methane monooxygenase (pMMO, EC 1.14.18.3) of Methylococcus capsulatus (Bath) and that of Methylosinus trichosporium OB3b ?
-
?
additional information Nitrosomonas europaea ATCC 19718 enzyme AMO-Ne in whole cells produces 1- and 2-alcohols from C4-C8n-alkanes, the regioselectivity is dependent on the length of the carbon chain. 2-Alcohols produced from C4-C7n-alkanes are predominantly either the R- or S-enantiomers, while 2-octanol produced from n-octane is racemic. AMO-Ne can discriminate between the prochiral hydrogens at the C-2 position, with the degree of discrimination varying according to the n-alkane, and AMO-Ne shows a distinct ability to discriminate between the orientation of n-butane and n-pentane in the catalytic site, as compared to the particulate methane monooxygenase (pMMO, EC 1.14.18.3) of Methylococcus capsulatus (Bath) and that of Methylosinus trichosporium OB3b ?
-
?
NH3 + a reduced acceptor + O2 Nitrosomonas europaea
-
NH2OH + an acceptor + H2O
-
?
NH3 + a reduced acceptor + O2 Nitrosomonas europaea ATCC 19718
-
NH2OH + an acceptor + H2O
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information alcohol productions in C5-C8 n-alkane oxidtions by enzyme AMO-Ne, overview Nitrosomonas europaea

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea Q04507 AND Q04508 subunits a and b
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Nitrosomonas europaea ATCC 19718 Q04507 AND Q04508 subunits a and b
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme AMO-Ne in whole cells produces 1- and 2-alcohols from C4-C8n-alkanes, the regioselectivity is dependent on the length of the carbon chain. 2-Alcohols produced from C4-C7n-alkanes are predominantly either the R- or S-enantiomers, while 2-octanol produced from n-octane is racemic. AMO-Ne can discriminate between the prochiral hydrogens at the C-2 position, with the degree of discrimination varying according to the n-alkane, and AMO-Ne shows a distinct ability to discriminate between the orientation of n-butane and n-pentane in the catalytic site, as compared to the particulate methane monooxygenase (pMMO, EC 1.14.18.3) of Methylococcus capsulatus (Bath) and that of Methylosinus trichosporium OB3b Nitrosomonas europaea ?
-
?
additional information analysis of selectivity of ammonia monooxygenase from Nitrosomonas europaea (AMO-Ne) for the oxidation of C4-C8n-alkanes to the corresponding alcohol isomers, ability of enzyme AMO-Ne to recognize the n-alkane orientation within the catalytic site, overview Nitrosomonas europaea ?
-
?
additional information enzyme AMO-Ne in whole cells produces 1- and 2-alcohols from C4-C8n-alkanes, the regioselectivity is dependent on the length of the carbon chain. 2-Alcohols produced from C4-C7n-alkanes are predominantly either the R- or S-enantiomers, while 2-octanol produced from n-octane is racemic. AMO-Ne can discriminate between the prochiral hydrogens at the C-2 position, with the degree of discrimination varying according to the n-alkane, and AMO-Ne shows a distinct ability to discriminate between the orientation of n-butane and n-pentane in the catalytic site, as compared to the particulate methane monooxygenase (pMMO, EC 1.14.18.3) of Methylococcus capsulatus (Bath) and that of Methylosinus trichosporium OB3b Nitrosomonas europaea ATCC 19718 ?
-
?
additional information analysis of selectivity of ammonia monooxygenase from Nitrosomonas europaea (AMO-Ne) for the oxidation of C4-C8n-alkanes to the corresponding alcohol isomers, ability of enzyme AMO-Ne to recognize the n-alkane orientation within the catalytic site, overview Nitrosomonas europaea ATCC 19718 ?
-
?
NH3 + a reduced acceptor + O2
-
Nitrosomonas europaea NH2OH + an acceptor + H2O
-
?
NH3 + a reduced acceptor + O2
-
Nitrosomonas europaea ATCC 19718 NH2OH + an acceptor + H2O
-
?
NH3 + duroquinol + O2
-
Nitrosomonas europaea NH2OH + duroquinone + H2O
-
?
NH3 + duroquinol + O2
-
Nitrosomonas europaea ATCC 19718 NH2OH + duroquinone + H2O
-
?

Synonyms

Synonyms Comment Organism
AMO
-
Nitrosomonas europaea
AMO-Ne
-
Nitrosomonas europaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Nitrosomonas europaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Nitrosomonas europaea

Cofactor

Cofactor Comment Organism Structure
duroquinol artificial Nitrosomonas europaea