Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.39 extracted from

  • Ensign, S.A.
    Hyman, M.R.; Arp, D.J.: In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper (1993), J. Bacteriol., 175, 1971-1980.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.39

Activating Compound

Activating Compound Comment Organism Structure
Serum albumin stimulates in vitro Nitrosomonas europaea
spermine stimulates in vitro Nitrosomonas europaea

General Stability

General Stability Organism
loss of AMO activity upon lysis of Nitrosomonas europaea results from the loss of copper from the enzyme, generating a catalytically inactive, yet stable and activable, form of the enzyme Nitrosomonas europaea

Inhibitors

Inhibitors Comment Organism Structure
Acetylene two populations of AMO in cell extracts. The low, copper-independent (residual) AMO activity is completely inactivated by acetylene in the absence of exogenously added copper. The copper-dependent (activable) AMO activity is protected against acetylene inactivation in the absence of copper. In the presence of copper both populations of AMO are inactivated by acetylene Nitrosomonas europaea

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the addition of CuCl2 to cell extracts results in 5- to 15-fold stimulation of ammonia-dependent O2 consumption, ammonia-dependent nitrite production, and hydrazine-dependent ethane oxidation. Two populations of AMO in cell extracts. The low, copper-independent (residual) AMO activity is completely inactivated by acetylene in the absence of exogenously added copper. The copper-dependent (activable) AMO activity is protected against acetylene inactivation in the absence of copper. However, in the presence of copper both populations of AMO are inactivated by acetylene Nitrosomonas europaea
MgCl2 stimulates in vitro. Loss of enzyme activity upon lysis of Nitrosomonas europaea results from the loss of copper from the enzyme, generating a catalytically inactive, yet stable and activable, form of the enzyme Nitrosomonas europaea
additional information Zn2+, Co2+, Ni2+, Fe2+, Fe3+, Ca2+, Mg2+, Mn2+, Cr3+, and Ag+, are ineffective at stimulating AMO activity Nitrosomonas europaea

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ammonia + AH2 + O2
-
 Nitrosomonas europaea NH2OH + A + H2O
-
 ?

Synonyms

Synonyms Comment Organism
AMO
-
 Nitrosomonas europaea